Expression of c-maf and mafB genes in the skin during rat embryonic development. Acta Histochem. 106, 65 - 67.Ogata A , Shimizu T , Abe R , Shimizu H , Sakai M . 2004 . Expression of c-maf and mafB genes in the skin during rat embryonic development . Acta Histochem 106 : 65 –...
在HEK293T细胞中转染MafA,MafB和OTUB1后,IP结果显示OTUB1可以显著抑制MafA的泛素化,但对MafB的泛素化没有影响.(4)在HEK293T细胞中过表达不同浓度的OTUB1和MafA,MafB,c-Maf,WB结果发现OTUB1可以显著上调MafA和c-Maf的蛋白水平,对MafB没有显著影响;在MM细胞株中敲低OTUB1的表达后,发现c-Maf的蛋白水平降低.而...
随着usp5质粒转染量的提高以及转染时间的延长,c-maf蛋白水平逐渐提高,提示usp5对c-maf的降解具有抑制作用,并使c-maf蛋白的半衰期延长;4.usp5也可使mafb的蛋白水平提高,半衰期延长;但是其对mafa的蛋白水平和半衰期没有明显变化,提示usp5对c-maf的作用具有一定程度的选择性和特异性;5.USP5使c-Maf下游靶基因CCND2...
值得注意的是,cMUL不能介导MafB和MafA的泛素化降解,说明cMUL具有特异性。此外,我们通过一系列的基因定向突变,构建了多个c-Maf的赖氨酸突变体,发现c-Maf的泛素化与第85和第350号位的赖氨酸有关,但是c-Maf泛素化的并不完全依赖这两个赖氨酸残基,因为K85和K350突变后,c-Maf仍然能够发生泛素化降解。在本研究中,...
AP-1 为一类含有碱性亮氨酸拉链的转录因子家族,由 Jun 蛋白家族(c-Jun、JunB 和 JunD)、Fos 蛋白家族(c-Fos、FosB、Fra1 和 Fra2)、ATF 蛋白家族(ATF2、ATF3/LRF1、B-ATF、JDP1、JDP2)和 Maf 蛋白家族(c-Maf、MafA、MafB、MafF/G/K 和 Nrl)中的蛋白亚基形成同源或异源二聚体。AP-1 在浆细胞...
of c-Maf in both HEK293T cells and MM cells. When OTUB1 was knocked down by shRNA, c-Maf ubiquitination level could be partly recovered. Notably, although MafA and MafB belong to the Maf family with c-Maf, OTUB1 decreased the ubiquitination level of MafA but had no effect on MafB....
Notably, MafA and MafB are also members of the c-Maf family, however, USP5 failed to deubiquitinate MafA, suggesting its substrate specificity. In the functional studies, USP5 was found to promoted the transcriptional activity of c-Maf. Consistent with the high level of c-Maf protein in MM...
MAF/TIGER Accuracy Improvement Project Mafa Mafa Mafa MAFA-2F1 MAFA-L MAFA-LIKE MAFA-like receptor MAFAA Mafac MAFAL Mafalda MAFAP MAFAR MAFARH MAFATDS MAFB MAFBA MAFBNZ MAFbx MAFC MAFCA MAFCAS MAFCO MAFCS MAFCU MAFD MAFD1 MAFDC ▼...
MafB takes part in strong transcriptional activity, while the acidic and hinge regions (AH) of c-Maf collectively serve as a strong transactivation domain... KY T Yoshida - 《Genes to Cells》 被引量: 162发表: 2002年 Novel DNA binding specificities of a putative herpesvirus bZIP oncoprotein. ...
Domain-swapping experiments reveal that the bZIP region of MafB takes part in strong transcriptional activity, while the acidic and hinge regions (AH) of c-Maf collectively serve as a strong transactivation domain. The AH region of L-Maf (but not c-Maf) conferred transactivation activity to ...