c-maf genemultiple myelomaRNAiproliferation目的:探讨c-maf基因对多发性骨髓瘤细胞增殖,侵袭力的影响.方法:脂质体法用c-maf siRNA转染多发性骨髓瘤细胞系RPMI8226,RT-PCR技术检测c-maf基因的表达,MTT实验和Transwell小室分别检测多发性骨髓瘤细胞增殖活性和侵袭力的改变,流式细胞术检测细胞周期,Wester
MAF (MAF BZIP Transcription Factor) is a protein coding gene located on human chromosome 16q23.2. MAF is also known as CCA4, AYGRP, c-MAF and CTRCT21. The human MAF gene encodes a 38492 Da protein containing 373 amino acids. The MAF protein is ubiquitously expressed in kidney, endometri...
Keywords:c—mafgene,multiplemyeloma,RNAi,proliferation,invasion,apoptosis 多发性骨髓瘤(multiplemyeloma,MM)约 占血液 系统疾病的 10%,是起 源于 B淋 巴细胞的恶性肿瘤。 新 型的靶 向治疗药物 ,如蛋 白酶体抑制剂 硼替佐米 在提高缓解率 、改善生活质量及延长患者寿命 方面 ...
Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010] 基因名: MAF 别名: CCA4,AYGRP,c-MAF,CTRCT21 基因ID: 4094 Chromosome:(GRCh37) 16 Start: 79627745 End: 79634622 Strand: 信号通路: MAF 基因突变与药物...
One day before transfection, HEK293T cells were seeded in six-well plates, when grown to 50% confluence, cells were subjected to gene delivery using polyethyleneimine (PEI) as described previously.11 Immunoblotting. After transfection with appropriate plasmids, HEK293T cells were lysed on ice in...
Gene ID (NCBI) 4094 RRID AB_10863127 偶联类型 Unconjugated 形式 Liquid 纯化方式 Antigen affinity purification UNIPROT ID O75444 储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. 储存条件 Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20...
c-Maf, is known as a Th2 specific transcription factor that could transactivate IL-4 gene expression. Recent studies have shown that c-Maf could transactivate Il21 and Il10 in Th17 cells and type 1 regulatory T cells (Tr1), respectively. However, the function of the tyrosine phosphorylated ...
Full size image To examine whether the iTregcell-dominant differentiation ofH. hepaticus-specific T cells is altered during intestinal inflammation, we co-transferred naive HH7-2tg and control 7B8tg T cells into colonizedIl10−/−recipients. Notably, only a small proportion of the transferred...
To generate RNA probes for in situ hybridization of whole mount and sectioned embryos, two ORF regions (−5 to 402 and 753 to 1127) of the c-maf gene were subcloned, and sense and antisense probes were synthesized using the T3 and T7 RNA polymerases with (digoxigenin)-UTP labeling kit...
Entrez Gene ID:(Human) 4094 功能 transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific bindingprotein bindingsequence-specific DNA bindingbasic leucine zipper transcription factor 参与通路 negative regulation of transcription from RNA polymerase II promotercytokine...