注意:为了便于后面分析结果,我们一般会选用已确定分子量大小而且纯度高的抗体作为marker与一抗同时孵育。 c-fos抗体c-fos免疫标记,免疫组化实验操作步骤(以石蜡切片为例) 仪器、设备: 移液枪、免疫组化笔、水浴锅、计时器、孵育湿盒、切片架、盖玻片、光学显微镜、洗瓶等。
c-fos expression as a marker of central cardiovascular neu- rons. Biol. Signals 4, 117e123.Dun NJ, Dun SL, Shen E, Tang H, Huang R, and Chiu TH. c-Fos expression as a marker of central cardiovascular neurons. Biol Signals 4: 117-123, 1995....
通俗的说,c-Fos在哪个脑区表达增多,就说明哪个脑区激活了,从而说明这个脑区参与了某种行为。比如实验中诱发小鼠焦虑抑郁,80-90分钟后杀掉小鼠,灌流,c-fos染色,哪个脑区表达增多,说明这个脑区参与焦虑抑郁的调控。刚才搜了一下“维基百科”,看看这上面是怎么描述的。 (structure...
并利用c-Fos抗体进行全脑免疫荧光标记,采用LS18平铺光片显微镜对样本进行3D成像,视频展示了小鼠脑c-Fos信号的分布以及经过Allen脑图谱配准后,提取纹状体区域内的c-Fos信号(Video 1),具体的脑图谱分析结果展示我们将会在后续文章中详细介绍。
et al. Expression of c-fos-like protein as a marker for neuronal activity following noxious stimulation in the rat. J. Comp. Neurol.,1990, 296(4), 517-530 [3] Fernando Cruz-Mendoza. et al. Immediate Early Gene c-fos in the Brain: Focus on Glial Cells. Brain Sci., 2022(5), 12...
上海玉博生物科技有限公司 主要致力于“C-fos(i mmediate early gene, ieg) 即刻早期基因(是一种原癌基因)抗原 yb-0469P”的生产销售。多年的“C-fos(i mmediate early gene, ieg) 即刻早期基因(是一种原癌基因)抗原 yb-0469P”生产与销售的经验,与各行业新老用户建立了
[2] Bullitt, E. et al. Expression of c-fos-like protein as a marker for neuronal activity following noxious stimulation in the rat. J. Comp. Neurol.,1990, 296(4), 517-530 [3] Fernando Cruz-Mendoza. et al. Immediate Early Gene c-fos in the Brain: Focus on Glial Cells. Brain Sci...
c-fos as a transcription factor acts in conjunction with other proteins to induce transcription of a wide variety of genes. c-fos expression is used as a marker of functional activity in the brain. c-fos expression is associated with tumour progression. ...
c-FOS is a protein derived from immediate early gene expression and is recognized as aspecific marker of neuronal activitythroughout the mammalian brain. Through a variety of perturbations (e.g. drug treatments, external stimuli), expression levels become elevated. By detecting c-FOS+ cells throug...
我染这个蛋白做神经元激活marker筛选焦虑相关脑区,但是使用的基因背景出现焦虑行为的小鼠,没有给外源刺激,虽然行为学上十字高架出现了差异,可是筛选焦虑相关的脑区的cfos信号却一直不稳定。一点成果都没有,好急好想哭。 常会出现一点阳性信号都没有脑片,救命有没有人做过,或者做环路的可以救救孩子😭😭😭😭...