Positive GDH or toxin results corresponded to lower PCR cycle threshold values (P?P?=?0.05) and diarrhea (91.0% vs. 79.4%, P?P?=?0.004) or laxative use (24.6% vs 36.1%, P?=?0.02). Toxin positivity was associated with antibiotic use (P?P?>?0.05). The application of the 2-step ...
Laboratory tests include detection of C.difficile common antigen (i.e., glutamate dehydrogenase), cell culture tests for Toxin A and/or Toxin B, EIA for Toxin A and /or B, bacterial culture and PCR (1). We evaluated a new screening test, the C.DIFF CHEKTM, in two formats, the C....
associated with CDI recurrence was the use of enzyme immunoassay (EIA) testing vs. PCR for initial CDI diagnosis (p= 0.046). This finding is consistent with analyses of the parent study, and may be due to higher-false positive rates or detection of non-toxin elaboratingC. difficilestrains wi...
difficile glutamine dehydrogenase (GDH) using the EIA C. DIFF CHEKTM test (Tech Lab, U.S.). Microbial populations were allowed to reach equilibrate growth before a single dose of ~107 cfu/mL C. difficile strain 210 spores (prepared following the method of Buckley, Spencer17) were added to...
First, samples were tested for glutamate dehydrogenase antigen and toxin A/B antigen using the C. DIFF QUIK CHEK COMPLETE® test (Alere North America, Inc.). Samples were considered positive if both GDH and toxin antigens were detected. Those that were inconclusive by this method (GDH ...
ImmunocompromisedDiagnosing Clostridioides (Clostridium) difficile infection is challenged by lack of a clear gold standard. We sought to determine if the two-step algorithm (screening GDH and toxin lateral flow assay followed by tcdB PCR) would have adequate clinical performance at a tertiary care...
doi:10.1016/j.diagmicrobio.2018.12.010Zuhha AshrafElham RahmatiJeffrey M. BenderNeha NandaRosemary C. SheElsevier
DIFF QUIK CHEK COMPLETE (Techlab), an enzyme immunoassay–based test with results for the glutamate dehydrogenase antigen (GDH) and C difficile toxin A/B. Hospital-onset (HO) CDI and CDI antibiotic use before and after testing were tracked. Ct values were obtained from the Infectious Diseases...
diff Quik Chek Complete kit immunoassay; GDH, Glutamate dehydrogenase; QGP, QIAstat-Dx Gastrointestinal Panel 2 PCR test; XCD, Xpert C. difficile PCR test. Algorithm 1, CDQ arbitrated by XCD; Algorithm 2, CDQ arbitrated by QIAstat-Dx Multiplex PCR; Algorithm 3, XCD followed by toxin A/B ...
A C. diff quick check complete® test (Abbott, Wavre, Belgium) for toxins and glutamate dehydrogenase antigen detection (GDH) was performed on each faeces sample (see Table 1). Confirmation with a specific qPCR test using 16S rDNA on DNA extracted from faeces was performed with the ...