difficile glutamate dehydrogenase (GDH) and C. difficile toxins A and B, followed by reflex testing of samples having inconclusive results (GDH positive and toxin negative) with PCR for identification of toxin gene specific DNA. The goal of this study was to compare the outcome and efficiency ...
Of 150 PCR-positive specimens, 52 (34.7%) were toxin-positive and 126 (84.0%) were GDH positive. Positive GDH or toxin results corresponded to lower PCR cycle threshold values (P<0.01). PCR-positive patients had more frequently documented antibiotic usage (78.4% vs 66.9%, P=0.05) and ...
associated with CDI recurrence was the use of enzyme immunoassay (EIA) testing vs. PCR for initial CDI diagnosis (p= 0.046). This finding is consistent with analyses of the parent study, and may be due to higher-false positive rates or detection of non-toxin elaboratingC. difficilestrains wi...
ME, U.S.) and used in all of the gut model experiments, strain R20291 (BI/NAP1/PCR ribotype 027/toxinotype III) was originally isolated in 2004 during an outbreak at the Stoke
First, samples were tested for glutamate dehydrogenase antigen and toxin A/B antigen using the C. DIFF QUIK CHEK COMPLETE® test (Alere North America, Inc.). Samples were considered positive if both GDH and toxin antigens were detected. Those that were inconclusive by this method (GDH ...
ImmunocompromisedDiagnosing Clostridioides (Clostridium) difficile infection is challenged by lack of a clear gold standard. We sought to determine if the two-step algorithm (screening GDH and toxin lateral flow assay followed by tcdB PCR) would have adequate clinical performance at a tertiary care...
The presence of GDH (Glutamate Dehydrogenase Enzyme) and toxin B was assessed using a chromatographic immunoassay (CerTest, Biotec, Spain), following the manufacturer s instructions. The confirmation of the presence of C. difficile DNA was performed by detection of a species-specific internal ...
Positive GDH or toxin results corresponded to lower PCR cycle threshold values (P?P?=?0.05) and diarrhea (91.0% vs. 79.4%, P?P?=?0.004) or laxative use (24.6% vs 36.1%, P?=?0.02). Toxin positivity was associated with antibiotic use (P?P?>?0.05). The application of the 2-step ...
DIFF QUIK CHEK COMPLETE (Techlab), an enzyme immunoassay–based test with results for the glutamate dehydrogenase antigen (GDH) and C difficile toxin A/B. Hospital-onset (HO) CDI and CDI antibiotic use before and after testing were tracked. Ct values were obtained from the...
GDH detection by both commercial tests showed high sensitivity (100%) and specificity (92.1%). On the other hand, toxin-based methods showed a sensitivity between 19.2 and 57.7%. In conclusion, the results suggest that rapid tests for GDH detection are not only suitable for CDI diagnosis as ...