dif- ficile toxin gene in GDH positive toxin negative samples. Ann Clin Lab Sci. 2012; 42(4): 397-400.Agaronov, Maksim et al. "Comparison of Genexpert PCR to BD Geneohm for Detecting C. difficile Toxin Gene in GDH Positive Toxin Negative Samples." Annals of Clinical and Laboratory ...
DIFF QUIK CHEK COMPLETE® test (Alere North America, Inc.). Samples were considered positive if both GDH and toxin antigens were detected. Those that were inconclusive by this method (GDH antigen positive, toxin negative) were reflexed to a molecular detection method which evaluated the ...
α微管蛋白乙酰转移酶1英文全称glutama dehydrogenase英文简称GDH 检测范围:8598nmol/L-1000pg/mL α-葡萄糖苷酶C英文全称liver kidney microsome autoantibody 1英文简称LKM-1 检测范围:4318nmol/L- α葡萄糖苷酶2英文全称rGT英文简称rGT 检测范围:4505nmol/L-1000pg/mL ...
METHODS. In October 2022, our system began reflex testing all PCR-positive stool samples with the C. DIFF QUIK CHEK COMPLETE (Techlab), an enzyme immunoassay–based test with results for the glutamate dehydrogenase antigen (GDH) and C difficile toxin A/B. Hospital-onset (HO) CDI and CDI ...
diff Quik Chek Complete (Techlab Inc.) and Immunocard (Meridian Bioscience) using remnant stool samples from 262 patients with suspected CDI. RESULTS. Sofia 2 demonstrated high agreement with the EIA methods for GDH (positive percentage agreement (PPA): 100%, negative percentage agreement (NPA):...
difficile common antigen by the C. DIFF CHEKTM test and/ or by PCR were positive for toxin B by the TC assay. Although a GDH-positive/TC-negative result may indicate growth of nontoxigenic isolates of C. difficile in the patient, we cannot rule out the possibility that some of these ...
Positive GDH or toxin results corresponded to lower PCR cycle threshold values (P?P?=?0.05) and diarrhea (91.0% vs. 79.4%, P?P?=?0.004) or laxative use (24.6% vs 36.1%, P?=?0.02). Toxin positivity was associated with antibiotic use (P?P?>?0.05). The application of the 2-step ...
GDH detection by both commercial tests showed high sensitivity (100%) and specificity (92.1%). On the other hand, toxin-based methods showed a sensitivity between 19.2 and 57.7%. In conclusion, the results suggest that rapid tests for GDH detection are not only suitable for CDI diagnosis as ...
Positive GDH or toxin results corresponded to lower PCR cycle threshold values (P<0.01). PCR-positive patients had more frequently documented antibiotic usage (78.4% vs 66.9%, P=0.05) and diarrhea (91.0% vs. 79.4%, P<0.01) and less frequent alternate etiologies of diarrhea (27.3% vs. 41.1...
The C.DIFF CHEKTM is an enzyme immunoassay for use as a screening test to detect C. difficile antigen, glutamate dehydogenase,in fecal samples. The goals of this evaluation were to determine the sensitivity, specificity, positive predictivevalue, negative predictive value and correlation of the ...