1.RNA-seq(4):Hisat2+FeatureCounts+DESeq2流程+作图! https://pzweuj.github.io/2018/07/18/rna-seq-4.html 2.一个植物转录组项目的实战 http://www.bio-info-trainee.com/2809.html 3.RNA_seq(1)植物转录组差异基因分析简单练习 https://www.jianshu.com/p/7146d5c41294 Part I II 基本是照着...
3.RNA_seq(1)植物转录组差异基因分析简单练习 https://www.jianshu.com/p/7146d5c41294 1.Volcano DE 绘图 2.PCA 绘图: 3.Heatmap:实现基因表达模式可视化的需求。 从这里可以看到这4个样本的表达差异,基本是一致的pattern,因为都是拟南芥的rna-seq 样本,no more difference. Rscript: library(pheatmap) se...
Single-cell sequencing is frequently affected by “omission” due to limitations in sequencing throughput, yet bulk RNA-seq may contain these ostensibly “omitted” cells. Here, we introduce the single cell trajectory blending from Bulk RNA-seq (BulkTrajBlend) algorithm, a component of the OmicVer...
该存储库包含两种基本形式的基本批量RNA-seq管道的演示,即Snakemake (在workflow/目录中)和bash脚本(在bash_workflow/目录中)。 这两个工作流程执行相同的分析,但是Snakemake管道更加健壮和推荐,尽管目前还不够完善。 这个Snakemake管道是作为教程的一部分而创建的,因此避免了使用某些Snakemake的完整/更复杂的实用程序。
RNA sequencing (RNAseq) can reveal gene fusions, splicing variants, mutations/indels in addition to differential gene expression, thus providing a more complete genetic picture than DNA sequencing. This most widely used technology in genomics tool box has evolved from classic bulk RNA sequencing (RNA...
Bulk RNA-seqhttps://www.youtube.com/watch?v=UgslMZ74ums Cellar heterogeneity细胞异质性的原因是morphology&genome&transcription&proteome,Cellar heterogeneity细胞异质性导致tumor。对于tumor使用PD1/PDL1无效,现在依据个性化需要使用single cell analysis,人群队列。
et al. Comprehensive analysis of bulk, single-cell RNA sequencing, and spatial transcriptomics revealed IER3 for predicting malignant progression and immunotherapy efficacy in glioma. Cancer Cell Int 24, 332 (2024). https://doi.org/10.1186/s12935-024-03511-1 Download citation Received21 May 2024...
Eoulsan was initially developed for bulk RNA-seq data, but the transcriptomics applications have recently widened with the advent of long-read sequencing and single-cell technologies, calling for the development of new workflows. Result We present Eoulsan 2, a major update that (i) enhances the...
Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3′ cDNA libraries for dozens of sa
title:Integrated analysis of single-cell and bulk RNA sequencing data reveals a pan-cancer stemness signature predicting immunotherapy response author: author time: 2022.04 journal: genome medicine(IF = 15) Objective: 癌症干细胞可能是 ICI 耐药的潜在原因,但缺乏直接的临床证据。