早!今天小编和大家分享一篇22年11月发表在Frontiers in Immunology(IF:8.786)杂志的文章《Identification and validation of a signature based on macrophage cell marker genes to predict recurrent miscarriage by integrated analysis of single-cell and bulk RNA-sequencing》。作者通过单细胞和bulk RNA测序的联合分析...
Bulk RNA-sequencing pipeline流程(含代码) 对于Bulk RNA-seq测序(用于比较转录组学,如不同物种的同种组织样本,也就是我们常说的常规转录组测序,注意和单细胞测序区分),我们常用的分析流程有很多,之前的文章也有介绍。偶然在github上发现一个总结的很完整的分析流程和大家分享。 我们依照作者的思路来进行: 使用NCBI ...
英文标题:Development of a CD8+ T cell associated signature for predicting the prognosis and immunological characteristics of gastric cancer by integrating single-cell and bulk RNA-sequencing期刊:Sci Rep. 2024 Feb 24;14(1):4524.影响因子:2区4.6DOI:10.1038/s41598-024-54273-9.研究领域:免疫治疗;...
paves the way 铺平道路 The development of single-cell RNA sequencing (scRNA-Seq) enables us to dissect gene expression at single-cell resolution and identify novel biomarkers with better performance(单细胞的优势) 以上均个人观点,欢迎讨论。禁止转载,如有需要,请简信联系作者!
Objective: This study amied to investigate the prognostic characteristics of triple negative breast cancer (TNBC) patients by analyzing B cell marker genes based on single-cell and bulk RNA sequencing. Methods: Utilizing single-cell sequencing data from TNBC patients, we examined tumor-associated B ...
RNA sequencing scRNA-seq: Single-cell RNA sequencing TME: Tumour microenvironment TFs: Transcription factors PPARG: Peroxisome proliferator-activated receptor-γ SCENIC: Single-cell regulatory network inference and clustering PD-1: Programmed cell death protein 1 RAS: Regulon activity scores ...
Analysis of cell subsets of single-cell RNA sequencing (scRNA-seq) from Breast Cancer (BC) patients. A. All the cell samples showed no significant batch effect; B. UMAP plot represents 15 cell clusters from 14 BC patients; C. The average expression of cell type marker genes in eight diffe...
Fig. 10. Sc-RNA sequencing analysis reveal the PAM.score on single cell level. (A) The distribution of 9 PAM regulators in eight cell types. (B) The different of 9 PAM regulators between normal cells and LUAD cells. (C) The different of PAM.score between normal cells and LUAD cells...
RNA-sequencing (RNA-seq) has become an increasingly cost-effective technique for molecular profiling and immune characterization of tumors. In the past decade, many computational tools have been developed to characterize tumor immunity from gene expression data. However, the analysis of large-scale RNA...
scRNA-seq, single-cell RNA sequencing; TME, tumor immune environment; PDAC, pancreatic ductal adenocarcinoma; UMAP, uniform manifold approximation and projection; GSEA, gene set enrichment analysis; TMGs, T cell marker genes; TCGA; The Cancer Genome Atlas; TMGS, T cell marker genes score; Tcm...