For example: Mix 20ml of melted, redistilled phenol with 20ml (an equal volume) of Tris/SDS buffer. To the resulting 40ml of Tris/SDS-saturated phenol, add 40ml (an equal volume) of chloroform:isoamyl alcohol, previously mixed in a ratio of 24:1. This is the extracting buffer, the sta...
The use of feathers as a sample of DNA isolation or DNA genome sources minimizes the stress on the chicken and simplifies sampling process of many birds. This study aimed to compare the concentration and purity of the DNA isolates from lysis buffer-Phenol Chloroform Isoamyl alcohol (PCI) and ...
laboratory practice. reagents in aqueous solution are prepared usingwater r. for liquid chromatography,water for chromatography ris used for the preparation of mobile phases when water, or an aqueous solution, is one of the components. where a reagent solution is described using an expression such ...