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2.3.2. Sample Clean-Up and Q-Exactive Analysis Peptide extracts were re-suspended in 0.05% (v/v) trifluoroacetic acid, 2% acetoni- trile and were analysed by LC-MS/MS using the Thermo Q-Exactive mass spectrometer coupled to a Dionex Ultimate 3000 RSLCnano [16]. Peptides were separated ...
Although the functions of these glycoproteins are not yet fully understood, plasma PAG-I concentrations have been used for pregnancy diagnosis and as a marker of placental/fetal well-being [6,21,24–27]. Water buffalo PAG-1 concentrations can be detected in the blood of pregnant females around...