2.3.2. Sample Clean-Up and Q-Exactive Analysis Peptide extracts were re-suspended in 0.05% (v/v) trifluoroacetic acid, 2% acetoni- trile and were analysed by LC-MS/MS using the Thermo Q-Exactive mass spectrometer coupled to a Dionex Ultimate 3000 RSLCnano [16]. Peptides were separated ...
All reagents were mixed and distributed into a 96-well qPCR plate before adding 4 µL of diluted cDNA (1:10 with water). Each sample was run in triplicate and the results were averaged. In parallel, to assess the non-specific amplicon (primer-dimer formation) or genomic DNA contamination...