The following antibodies were used: ant-γ-H2AX (phospho-histone H2AX [Ser139], 05-636 [Millipore]), anti-Rad51 (ab213 [Abcam]), anti-MED1 (A300-793A [Bethyl Laboratories]), anti-H3K27ac (07-360 [Millipore]), anti-CDK9 (sc-8338 [Santa Cruz]), and anti-Brd4 (A301-985A; ...
The lentiviral shRNA plasmids pLKO.1 targeting mouseBrd4(clone IDs TRCN0000311976, TRCN0000088480, and TRCN0000088481) were purchased from Sigma. Anti-RbBP5 (A300–109A), anti-Brd4 (A301–985A100), and anti-MED1 (A300–793A) were from Bethyl Laboratories. Anti-C/EBPα (sc-61X), anti...
Antibodies The following antibodies were used in ChIP assays, western blotting assays and for immunoprecipitation: anti-H3 (ab1791; Abcam, Cambridge, UK), anti- BRD4 (A301-985A; Bethyl Labs, Montgomery, TX, USA); anti-GFP (ab290; Abcam) and Rabbit polyclonal IgG (ab27472; Abcam). ...
Rabbit anti-Brd4 Bethyl Cat#: A301-985A Rabbit anti-phospho-Brd4 Millipore Cat#: ABE1451 Rabbit anti-P300 Santa Cruz Cat#: sc-585 Rabbit anti-MLL1 Bethyl Cat#: A300-374 Mouse anti-Gapdh Abcam Cat#: ab8245 Mouse anti-GluA1 Millipore Cat#: MAB2263 Rabbit anti-Shank2 Cell Signaling Cat...
Slides were incubated with BRD4 antibody from Bethyl Laboratories (Montgomery #A301-985A100, 1:2500), phospho-Y1007/Y1008-JAK2 from Abcam (#ab32101, 1:250 dilution), α-SMA antibody from Sigma (#A2547, 1:7500), or pBRD4 antibody (1:1000) overnight at 4 °C, followed by 5 ...
Rabbit polyclonal anti-BRD4 (human and mouse) (long isoform) Bethyl Laboratories Cat#: A301-985A50; RRID: AB_2631449 Rabbit polyclonal anti-BRD2 Bethyl Laboratories Cat#: A302-583A; RRID: AB_2034829 Rabbit polyclonal anti-BRD3 Bethyl Laboratories Cat#: A302-368A; RRID: AB_1907251 Rabbit po...
Rabbit monoclonal anti-BRD4 (WB and ChIP) Bethyl Lab Cat# A301-985A100; RRID: AB_2620184 Rabbit polyclonal anti-H4K5bu (WB and ELISA) PTM biolabs Cat#PTM-313 Rabbit polyclonal anti-H4K5bu (ChIP) PTM biolabs Cat#PTM-310 Rabbit polyclonal anti-H4K8bu (WB) PTM biolabs Cat#PTM-311 Ra...