品牌:Bethyl 货号:A301-985A 用途:详见说明书 亚型:见优宁维官网 是否单克隆:详见说明书 免疫原:见优宁维官网 保质期:详见说明书 CAS编号:见优宁维官网 标记物:详见说明书 包装规格:见优宁维官网 抗原来源:详见说明书 适应物种:见优宁维官网 克隆性:见优宁维官网 ...
Antibodies The following antibodies were used in ChIP assays, western blotting assays and for immunoprecipitation: anti-H3 (ab1791; Abcam, Cambridge, UK), anti- BRD4 (A301-985A; Bethyl Labs, Montgomery, TX, USA); anti-GFP (ab290; Abcam) and Rabbit polyclonal IgG (ab27472; Abcam). ...
Bethyl认识到BRD4在细胞周期调控和肿瘤发生中的重要性,研发了质量优秀的BRD4抗体,以及许多其他相关蛋白质的抗体,以促进癌症研究发展。 图1:IHC-IF 检测福尔马林固定石蜡包埋(FFPE)的人胃癌组织 BRD4 (黄色) 和 细胞角蛋白Cytokeratin (红色)的表达. 抗体: 重组单...
Antibodies and Chemicals The following antibodies were used: SPOP (ab137537; Abcam), SPOP (16750-1-AP; Proteintech), BRD2 (A302-583A; Bethyl), BRD2 (ab139690; Abcam), BRD3 (A302-368A; Bethyl), BRD4 (ab128874; Abcam), BRD4 (A301-985A; Bethyl), Myc (9E10; Sigma-Aldrich), Myc ...
The lentiviral shRNA plasmids pLKO.1 targeting mouseBrd4(clone IDs TRCN0000311976, TRCN0000088480, and TRCN0000088481) were purchased from Sigma. Anti-RbBP5 (A300–109A), anti-Brd4 (A301–985A100), and anti-MED1 (A300–793A) were from Bethyl Laboratories. Anti-C/EBPα (sc-61X), anti...
Rabbit polyclonal anti-BRD4 (human and mouse) (long isoform) Bethyl Laboratories Cat#: A301-985A50; RRID: AB_2631449 Rabbit polyclonal anti-BRD2 Bethyl Laboratories Cat#: A302-583A; RRID: AB_2034829 Rabbit polyclonal anti-BRD3 Bethyl Laboratories Cat#: A302-368A; RRID: AB_1907251 Rabbit po...
将抗brd4(a301-985a,bethyllabs)抗体(50μg)与100μlaminolink树脂(thermofisherscientific)偶联。将细胞裂解物样品(5mg)与预洗涤的免疫树脂在振荡器上于4℃下孵育2小时。在含有0.4%igepal-ca630的裂解缓冲液和不含去污剂的裂解缓冲液中洗涤珠,然后是两个150mmnacl的洗涤步骤。通过用lys-c和甘氨酸蛋白酶进行珠上...
Rabbit anti-Brd4 Bethyl Cat#: A301-985A Rabbit anti-phospho-Brd4 Millipore Cat#: ABE1451 Rabbit anti-P300 Santa Cruz Cat#: sc-585 Rabbit anti-MLL1 Bethyl Cat#: A300-374 Mouse anti-Gapdh Abcam Cat#: ab8245 Mouse anti-GluA1 Millipore Cat#: MAB2263 Rabbit anti-Shank2 Cell Signaling Cat...
Slides were incubated with BRD4 antibody from Bethyl Laboratories (Montgomery #A301-985A100, 1:2500), phospho-Y1007/Y1008-JAK2 from Abcam (#ab32101, 1:250 dilution), α-SMA antibody from Sigma (#A2547, 1:7500), or pBRD4 antibody (1:1000) overnight at 4 °C, followed by 5 ...
Antibodies The following antibodies were used in ChIP assays, western blotting assays and for immunoprecipitation: anti-H3 (ab1791; Abcam, Cambridge, UK), anti- BRD4 (A301-985A; Bethyl Labs, Montgomery, TX, USA); anti-GFP (ab290; Abcam) and Rabbit polyclonal IgG (ab27472; Abcam). ...