16天后加入25μl CellTiter-Glo 2.0试剂(Promega),振荡2分钟,室温孵育10分钟,用CLARIOstar酶标仪(BMG Labtech)检测发光值。 EPIC甲基化芯片及分析 详见补充方法文件。 流式细胞分选 细胞经胰酶消化后重悬于FACS缓冲液(含7%胎牛血清的DPBS),用碘化丙啶排除死细胞后分选单细胞至96孔板。GSK处理后的WEHI-CS62克隆...
BRCA2 Polyclonal antibody 19791-1-AP P53 Polyclonal antibody 10442-1-AP BARD1 Polyclonal antibody 22964-1-AP RAD51 Polyclonal antibody 14961-1-AP CHEK2 Polyclonal antibody 13954-1-AP ER Polyclonal antibody 21244-1-AP RBBP8 Polyclonal antibody ...
BRCA1/BRCA2基因热点突变检测试剂盒,BRCA1和BRCA2基因突变检测试剂盒 1、检测意义 BRCA基因的突变与家族性乳腺癌及卵巢癌的发生密切相关,突变导致基因的抑制肿瘤作用降低或消失,导致细胞恶变和肿瘤的发生。约5～10的乳腺癌和遗传易感性相关,目前发现
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. ...
BRCAl第20外显子下游第35个碱基处G>A (IvS20+35G>A),致使该位点一个精氨酸被一个组氮酸取 代。该位点未在相关文献或BIC网站(Breast Cancer Information Core) (http://www.nhgri.nih.gov/Intramural～ research/Labtransfer/Bi∽上查到,它们有可能是中国河北人 ...
2, 1183–1196 (1996). Google Scholar Narod, S. A. Modifiers of risk of hereditary breast and ovarian cancer. Nature Rev. Cancer 2, 113–123 (2002). Google Scholar Meijers-Heijboer, H. et al. Breast cancer after prophylactic bilateral mastectomy in women with a BRCA1 or BRCA2 mutati...
北京大学医学部细胞生物学实验室 本实验室主要从事抑癌基因,特别是乳腺癌易感基因BRCA1在维护基因组稳定性方面的作用及其抑癌机理研究。研究方向包括:(1)DNA损伤修复;(2)细胞周期和检验点、有丝分裂调控;(3)染色质重建和表遗传学。除此之外,肝癌细胞分子生物学也是本室的一个重要研究方向。
Among ERBB2-amplified tumors of different tissue origins, ERBB2/HOXB13 co-amp with a BRCA1 loss appeared to be enriched in breast cancer compared to other tumor types. Lastly, patients with ERBB2/HOXB13 co-amplified and BRCA1 lost tumors displayed a significantly shorter progression-free ...
本发明提供一种brca1/2基因的扩增子文库构建方法,所述方法包括对样本中包含brca1/2基因区域进行靶向扩增,所述靶向扩增包括1)以所述样本为模板,通过第一引物进行第一轮pcr扩增,2)以第一轮pcr扩增的产物为模板,通过第二引物进行第二轮pcr扩增,以及3)以第二轮pcr扩增产物为模板,通过第三轮pcr扩增构建brca1/2基因...
However, because Cas9Dand Cas9Hinduced a nick respectively on the target strand within the DNA-RNA hybrid and on the non-target single strand at the gHR1a, gHR1b, gHR2 and gHR3 target sites, the strand asymmetry in BRCA1-mediated suppression of LTGC bias may not be due to replication,...