InBrca1-deficient mESC, the delayed availability of the second ends would facilitate NHEJ due to inefficient HR (Supplementary Fig.6a). We thus used mESC harboring a single-copy NHEJ reporter previously established to test this possibility46. In this NHEJ reporter containing aGFPexpression cassette ...
InBrca1-deficient mESC, the delayed availability of the second ends would facilitate NHEJ due to inefficient HR (Supplementary Fig.6a). We thus used mESC harboring a single-copy NHEJ reporter previously established to test this possibility46. In this NHEJ reporter containing aGFPexpression cassette ...
To test whether NF-κB pathway activation could cause a proliferation advantage in hTert-HME1 cells, a constitutively activated form of the IκB-kinase β with S177E and S181E mutations (IKK-βEE) was expressed in the hTert-HME1 cell line (Figure 5B). IKK-βEE expression in EGF-free...
To test if PARP-trapping agents other than olaparib also induce SCEs, we treated RPE-1 cells with a panel of PARP inhibitors that trap PARP onto DNA with variable capacity23: olaparib, veliparib and talazoparib (Figs. 3B and S3A). When used at IC25 dose, all three PARP inhibitors ...
Code availability The CCA code is also available at Zenodo (https://doi.org/10.5281/zenodo.5149154). References Download references Author information Sylvie M. Noordermeer Present address: Department of Human Genetics, Leiden University Medical Center, Leiden, The Netherlands ...