All gene sets are significant to FDR < 0.01. (D) RNA-seq was performed 6 days after treatment with control or BRCA2 siRNAs (n = 4 in each group), and differentially expressed transcripts were identified and intersected with BRCA2-bound genes identified by ChIP-seq. Significance testing was...
we report genome-scale CRISPR–Cas9 synthetic lethality screens in isogenic pairs ofBRCA1- andBRCA2-deficient cells and identifyCIP2Aas an essential gene inBRCA1- andBRCA2-mutated cells. CIP2A is cytoplasmic in interphase but, in mitosis, accumulates at DNA lesions as part of a complex with ...
Using paired Cas9-sgRNA approach34, we generatedBrca1-deficient (Brca1m/m) mESC in which mutatedBrca1gene encodes unstable BRCA1 lacking the C-terminal BRCT repeats (Supplementary Fig.1d, e)35. Cas9-gB2 and nCas9-gB2 were transfected to induce a number of two-ended DSBs and nicks in iso...
Using paired Cas9-sgRNA approach34, we generatedBrca1-deficient (Brca1m/m) mESC in which mutatedBrca1gene encodes unstable BRCA1 lacking the C-terminal BRCT repeats (Supplementary Fig.1d, e)35. Cas9-gB2 and nCas9-gB2 were transfected to induce a number of two-ended DSBs and nicks in iso...