Alithea Genomics MERCURIUS BRB-Seq™ 试剂盒 Bulk RNA Barcoding followed by sequencing(批量RNA条形码和测序,BRB-seq™)是 Alithea 的专利技术。该技术对样品进行条形码标记,再集中到单管中统一处理,大幅减少试剂消耗及人工操作。 ◆MERCURIUS BRB-seq™试剂盒的主要特点 ● 第一次逆转录反应后,可立即集中在...
索取Ion Torrent 下一代测序技术详细技术资料请填写联系方式>> >> 原文检索:BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing (生物通:伍松)
RNA sequencing of isolated E retinal ECs was done using paired-end Illumina sequencing (NovaSeq 6000 System). Single-cell RNA sequencing (snRNA) of in vitro human retinal endothelial microvessel model was performed under low and high- glucose conditions. DESeq2 analysis was performed to identify ...
After sequencing your BRB-seq libraries, you should obtain two fastq files per library: R1 fastq file: This file should contain the barcodes and UMIs of the BRBseq construct. Usually, the barcode comes before the UMI sequence. And the UMI sequence is optional. ...
and the remaining 39 cases were used for validation.In addition, association between the predictive imaging biomarker and genomic profiles were assessed using RNA expression data of these matched patients obtained with next-generation RNAsequencing and RT-PCR.Results:All imaging features from two indepen...
基于胡家志课题组2019年开发的全面评估基因编辑安全性的方法PEM-seq (Primer-extension-mediated sequencing)[2],作者首先评估了新一代CAR-T技术的安全性。PEM-seq方法的详细操作指南也于2022年公开发表于Cell系列的杂志STAR protocols上(https://doi.org/10.1016/j.xpro.2021.101088)[3]。新一代CAR-T细胞的构建结合...
原文检索:BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing (生物通:伍松)
Deep sequencingBlue Rubber Bleb Nevus syndrome (Bean syndrome, BRBN) is a rare, severe disorder of unknown cause, characterized by numerous cutaneous and internal venous malformations (VMs); gastrointestinal lesions are pathognomonic. We discovered somatic mutations inTEK, the gene encoding TIE2, in...
Moreover, the plan is also inherently robust against sequencing and delivery errors. Therefore, it allows to reduce margins on the ITV. The method outperforms gating both in dose coverage as well as delivery robustness and increases the delivery efficiency.doi:10.1118/1.3182569...