To train and validate our model, we randomly sampled and VR-annotated 48 × 100³ voxel patches (referring to 5,889 cells) from a c-Fos-labeled brain. From these we trained a 3D BasicUNet (Extended Data Fig. 2d). In addition, we trained recent larger segmentation models, such as...
However, the observed distribution of barcode abundance is not perfectly uniform in our library, which means that cells are more likely to be labeled with some barcodes than with others. The expected number47,55 of non-uniquely labeled cells, E(X), is then given by: ...
Richard M. Leahy is Director of the Signal and Image Processing Institute at the University of Southern California and leads the BIG (Biomedical Imaging Group) Lab, which hosts the Brainstorm software. Richard has worked on problems related to brain ma...
Distortion of homeostatic signaling proteins by simulated microgravity in rat hypothalamus:A(16) O/(18) O-labeled comparative integrated proteomic approach. Proteomics, 2014; 14, 262-73. doi: 10.1002/pmic.201300337 [26] Iqbal J, Li W, Hasan M, et al. Differential expression of specific ...
(lab cultured resected tissue),in silico (computer models) and, in vitro.In vitro models are a bioengineering effort to mimic the tissue of interest based on the triad stem cells, extracellular matrix, and soluble factors. Applied to the development of in vitro neurovascularity unit, this ...
Each row corresponds to a striatum CoExMap module labeled by numeric label, number of genes, and module color. Each column corresponds to a heterozygous KO of the indicated gene, a cell type, or to diurnally variable genes. Color next to the gene symbol shows the striatum CoExMap module ...
The uptake of labeled palmitic, oleic, linoleic, and arachidonic acids, esterified or not in lysophosphatidylcholine, was also studied in brain, liver, heart, and kidney. Only the brain preferentially incorporated unsaturated (but not saturated) lysoPC, with the uptake increasing with the degree ...
Region of interest was calculated by tracing the outline of the desired structure, identified by the presence of nuclei stained with Hoechst 33258, on a digital picture captured using ImageJ system, which was also used to count the labeled cells. 2.9. NeuronJ measurement of DCX+ cells arbor...
While single-cell technologies have greatly advanced our comprehension of human brain cell types and functions, studies including large numbers of donors and multiple brain regions are needed to extend our understanding of brain cell heterogeneity. Integ
determined using the bicinchoninic acid method and frozen in aliquots at −80 °C. Protein mixture was digested overnight and diluted to obtain peptides. Peptides were labeled using TMTPro isobaric tags (Thermo Scientific). Subsequently, high pH fractionation was performed as described in ...