PCR was performed using 2X Q5 PCR master mix (NEB) and GoTaq master mix for colony PCR (Promega, Madison, WI, USA). Primers were purchased from IDT (Louvain, Belgium), and DNA fragments from Twist Bioscience or IDT. Plasmid extraction and DNA purification were performed using Monarch kits...
one mouse line with a single recombinase system, and three embryonic stem (ES) cell lines that are designed to study the way functional, molecular, and anatomical features relate to each other in building circuits that underlie physiology and behavior. As a proof-of-principle, we applied three ...
2C). This suggests that the iGenSig-Rx scores are positively associated with pCR rate but negatively correlated with ER and PR subtypes. The iGenSig-Rx scores did not show associations with treatment arms, menopausal status or tumor stage, which is consistent with the results of the CALGB ...
An IC-PCR method for detection of Cryptosporidium and Giardia in natural surface waters in Finland We developed an immunocapture-based polymerase chain reaction (PCR) assay for simultaneous detection of Cryptosporidium parvum oocysts and Giardia intestin... R Rimhanen-Finne,A H?Rman,P Ronkainen,...
Construction of the reporter vectors pBS3Kluxand pBS3Eluxwas performed by PCR amplification of pBS3Clux19with primers TM4185 and TM4186 to generate acat-free fragment. The newB. subtilisantibiotic resistance cassetteskananderm, were generated by using pDG78050and pDG64750as templates with primers ...
a PCR amplification was performed using a specific oligo dT-Anchor primer, provided by the kit, and a second nested reverse primers, with the AccuPrime™ Taq DNA Polymerase System (Thermo Scientific). A second PCR was performed using a specific PCR Anchor primer, provided by the kit, and ...
The ability of proteins and nucleic acids to undergo liquid–liquid phase separation has recently emerged as an important molecular principle of how cells rapidly and reversibly compartmentalize their components into membrane-less organelles such as the nucleolus, processing bodies or stress granules1,2....
Operating Principle Air Shower Type Type Electronic Chain External Size (W*D*H) mm 760*670*1100 Internal Size (W*D*H) mm 600*600*600 Material 304 Stainless Steel Interlock Electronical UV Lamp 15W*1 HEPA Filter Efficiency ≥99.995% at 0.3μm ...
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gcc编译器GCC程序设计Box-BenhnkenBoom BoxR-BoxBox-BehnkenG-boxBox-VellumBounding BoxMADS-boxTATA-boxGC-boxCAAT-boxW-boxBOX-PCRDEAD-boxHMG-box水滴试验 补充资料:black-box model 分子式: CAS号: 性质: 仅利用外部的、宏观的直接经验,未利用过程机理方面的信息及基本物理定律所建立的数学模型。通常由试验...