Using this platform, we engineer bi-specific Wnt mimetics that target Frizzled and low-density lipoprotein receptor-related proteins and evaluate their effects on bone accrual in murine models. These synthetic Wnt agonists induce rapid and robust bone building effects, and correct bone mass deficiency...
which might explain the reduced18F-FDG uptake in tibiae of our cold-exposed mice. However, histomorphometry of proximal and distal tibiae revealed no differences in adipocyte density or BM adiposity between the three groups (Supplementary Fig.4A–D)...
PEMF treatment significantly (p < 0.05, Fisher's exact test) increased the fusion rate at 6 and 12 months for certain high-risk subjects who had at least 1 clinical risk factor of being elderly, a nicotine user, osteoporotic, or diabetic; and for those with at least 1 clinical risk facto...
Interestingly, newly formed bone is disorganized, typically with low density, and is weak in strength [114,115]. This is due to a malalignment of osteoblasts along a collagen matrix, leading to the production of bone with a spongy structure as opposed to a hard and compact l...
Brown TT, McComsey GA, King MS, Qaqish RB, Bernstein BM, da Silva BA (2009) Loss of bone mineral density after antiretroviral therapy initiation, independent of antiretroviral regimen. J Acquir Immune Defic Syndr 51 (5):554–561. https://doi.org/10.1097/ QAI.0b013e3181adce44 23. ...
Here we report the identification of human CD66b−CD64dimCD115− neutrophil-committed progenitor cells (NCPs) within the SSCloCD45dimCD34+ and CD34dim/− subsets in the bone marrow. NCPs were either CD45RA+ or CD45RA−, and in vitro experiments s
Pancreatic islets were largely reduced in size with a decrease in cellular density. Although the treatment of diabetic rats with either MSCs or PIO restored normal histology of pancreatic islets that were associated with less vacuolation, normal-sized islets were not restored. Interestingly, ...
Cell cultures were seeded at a density of 1.5 × 104 cells in six-well plates and grown in Low Glucose DMEM medium (EuroClone, Milan, Italy) containing 10% FBS (Euro-Clone, Italy) and 3 ng/mL of b-FGF (Prepotech, London, UK), 1% penicillin/streptomycin (Microgem, Naples, Italy),...
MG-63 cells with a seeding density of 15.0 × 103 cells/cm2 were cultured until a subconfluent monolayer was reached with the following complete replacement of CGM with ceramic extracts. After 24 h, the viability of the cell culture was analyzed using the MTT assay [99]. 4.12. Evaluation ...
This biomaterial showed a density and microporosity similar to that of natural bone and, after treatment with simulated body fluid, exhibited a mechanical resistance to compression greater than that of native bone due to the induced mineralization of HA crystals on its surface [83]. Salehi et al...