Design: Using a series of tissue microarrays that contain 419 GIST and 63 gastrointestinal LMS with long term follow-up, we determined the density of the macrophages in each 0.6 mm tumor cores with the macrophage marker CD163 and examined its prognostic significance by Kaplan-Meier analysis. ...
Measurement of ALP activity. MG-63 or ROB cells were seeded in 12-well plates at a density of 2 × 105 cells per well. The following day, the cells were placed in 1% FBS-containing culture medium for 24 h and then treated with the same culture medium containing SAL (...
Firstly, A549 cells were planted in 6-well plates with a density of 2 × 106 cells per well and cultured for overnight to allow cells attachment. The cell culture medium was then replaced with fresh cell culture medium that contained free DOX or DOX loaded DOX-hyd-PEG-ALN micelle...
The data are expressed as the ratio of the optical density (OD) value of the treated group to the OD of the control group. Flow cytometry. The CXCR4 expression, apoptotic rate and cell cycle distribution of MSC were eval- uated using flow cytometry. After treatment with different ...
Design: Using a series of tissue microarrays that contain 419 GIST and 63 gastrointestinal LMS with long term follow-up, we determined the density of the macrophages in each 0.6 mm tumor cores with the macrophage marker CD163 and examined its prognostic significance by Kaplan-Meier analysis. ...
2.8. Small Interfering RNA Transfection For the small interfering RNA (si-RNA) transfection experiments, BMSCs were seeded in 12-well plates at a density of 2 × 104 cells/well. After inducing cell senescence by use of 10 g/L of D-gal for 48 h, according to the si-RNA instruction ...