[2] Jeong D.-E., McCoy M., Artiles K., Ilbay O., Fire A., Nadeau K., Park H., Betts B., Boyd S., Hoh R., et al. Assemblies of Putative SARS-CoV2 Spike Encoding mRNA Sequences for Vaccines BNT-162b2 and mRNA-...
The emergence of immune-escape variants of severe acute respiratory syndrome coronavirus 2 warrants the use of sequence-adapted vaccines to provide protection against coronavirus disease 2019. 严重急性呼吸道综合征冠状病毒2的免疫逃逸变种的出现,使得我们有必要使用序列适应性疫苗来提供对2019年冠状病毒疾病的保护。
We isolated and sequence-confirmed live Omicron virus from an infected person and observed that Omicronrequires the angiotensin-converting enzyme 2 (ACE2) receptor to infect cells. Wecompared plasma neutralization of Omicron relative to an ancestral SARS-CoV-2 strainand found thatneutralization of ...
This study evaluated the humoral immune response to CoronaVac™ (virus inactivated) and BNT162b2 (mRNA- based) vaccines in PLWH and HIV-negative controls, with and without a booster sequence. Methods We conducted a cross-sectional study on PLWH and HIV-negative controls who received CoronaVac ...
The data have been deposited in the Sequence Read Archive linked to BioProject accession number PRJNA775994. All other data needed to evaluate the conclusions in the paper are present in the paper or the Source Data files. Source data are provided with this paper. References Amanat, F. et al...
(Takara Bio) in combination with the NexteraXT DNA Library Preparation kit to append dual-indexed adaptor sequences (Illumina). Libraries were validated by capillary electrophoresis on an Agilent 4200 TapeStation, pooled at equimolar concentrations, and sequenced on an Illumina NovaSeq6000 at 100SR,...
The QIIME2 (v. qiime2-2021.11) [19] pipeline was used to process the generated raw FASTQ files. First, the paired-end sequences were imported and demultiplexed, and then, quality filtering was performed with the deblurring denoising-16S method [20], which was cut to a quality score of 30...
130 In brief, flat-bottomed high-binding 96-well Corning plates (NY, catalog 9018) were coated with 25 ng per well of SARS-CoV-2 wildtype sequence of recombinant RBD (GenBank MN975262.1, amino acids R319-K529) or 50 ng per well of recombinant spike (GenBank MN90894, amino acids M1...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is continuing to evolve around the world, generating new variants that are of concern on the basis of their potential for altered transmissibility, pathogenicity, and coverage by vaccines and therapeutic agents1,2,3,4,5. Here we show...
(n = 18) (b, d) were stimulated overnight with three overlapping peptide pools representing different portions of the wild-type sequence of SARS-CoV-2 S (N-terminal pools S pool 1 and RBD, and the C-terminal S pool 2), and analysed by flow cytometry (gating strategy in Supplementary ...