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The software Sequencer 4.0 was used to screen the SNP markers between parents (Dazao and S4. cot parents) and F1. The primers for the SNP markers are given in Supplementary information, Table Annotating Candidate Genes within the cot Linkage Region. We searched the cot-linked region in ...
Recombinant proteins of three mutants and wild type BmSUC1 were obtained by using a Bac-to-Bac/BmNPV expression system and BmN cells. Enzymatic activity, kinetic properties, and substrate specificity of the four proteins were analyzed. High Performance Liquid Chromatography (HPLC) was used to ...