Human T cells were isolated from buffy coats (Sanquin Blood Bank, Amsterdam, The Netherlands) using a Ficoll density gradient (F4375; Sigma-Aldrich) and negative selection kits (#130–096-533, # 130–096-495; Miltenyi Biotec, Bergisch Gladbach, Germany) [58]. Purity was tested using flow ...
To create a model of coagulation factor XIIa, we used a crystal structure from the protein bank PDB (Protein Data Bank) [28]. At the time of this study, five structures of factor XIIa had been found, obtained by X-ray structural analysis: 6QF7, 6GT6, 6B77, 6B74, 6L63. To buil...
performed using the Dako REAL EnVision Detection System, rabbit/mouse kit (K5007, DakoCytomation, Glostrup, Denmark), and treated with 0.3% Peroxidase Block (DakoCytomation, Glostrup, Denmark) to block endogenous peroxidase activity, and then blocked with the host serum of a secondary antibody. Af...