PBMC were obtained by Ficoll-Isopaque density gradient separation of mononuclear cell enriched fraction (Hôtel Dieu, Blood Bank, Paris, France) from healthy adult defibrinated peripheral blood. Cultures were performed from thawed PBMC (usually containing >90% of viable cells as tested by trypan ...
Evelina London Children’s Hospital, Guy’s and St. Thomas’ NHS Foundation Trust, London SE1 7EH, UK 6 UCL Great Ormond Street Institute of Child Health, University College London, London WC1E 6BT, UK * Author to whom correspondence should be addressed. J...