4.Examine after 18-24 hours. Interpretation of Results Sheep Blood Agar can be used as a primary-plating medium. Primary isolation is performed to separate and isolate organisms present in a sample. This separation allows for characterization of colony types and may indicate the presence of ...
7. Interpretation of results Interpretation of positive blood culture results are challenging to both clinicians and microbiologists. With the background of blood culture contamination rates of up to half of all positive cultures and previously considered contaminants now more frequently implicated in dise...
The TTP of each BC was recorded, and liquid from each BC bottle was inoculated into a blood agar plate, a MacConkey agar plate, and a chocolate agar plate and incubated at 36°C for 18–24 h. An anaerobic Brucella culture medium was added to positive anaerobic bottles instead of positive...
Fresh CSF was dispensed into a 96-well plate at a volume of 200 µL/well, or stored at -20 ˚C for future use. The overnight S. suis culture was centrifuged and resuspended in PBS to reach a concentration of 3 × 108 CFU/mL. Then, S. suis were added to 96-well plate ...
the blood culture broths were checked for signs of bacterial growth (turbidity, hemolysis, and clot formation) daily for up to 7 days. Blood culture broth bottles that show these signs of growth were subcultured in the appropriate culture media, such as chocolate agar, blood agar, modified Tha...
The dilution series was plated from 10−3 to 10−10 onto NBTL agar plates (20 μl per 1/4 agar plate). The plates were then incubated at 28°C and checked regularly for CFU, as above. To try to understand the mechanisms underpinning the main findings, we conducted four additional ...
one culture plate with mixed growth (21 h group), located off the inoculation streaks in the 3rd and 4th quadrant of the chocolate agar plate Amplification of genetic material was noted in one blood culture bottle using an assay targetingStaphylococcusspp. Decision to use this assay was made wh...
Time-to-colonies was defined as time between incubation and recovery of bacterial colonies on agar plate. Results Blood culture results Between October 2017 and March 2020, a total of 3140 blood cultures were sampled from 3031 patients (Fig. 2). Fig. 2 Breakdown of blood culture data from ...
Interpretation of results. MALDI-TOF MS results and the identification obtained by conventional methods were correlated as follows: total concordance, MALDI-TOF and conventional methods provided the same identification at the species level; partial concordance, both methods provided a pathogen identification...
The blood–brain barrier (BBB) prevents harmful toxins from entering brain but can also inhibit therapeutic molecules designed to treat neurodegenerative diseases. Focused ultrasound (FUS) combined with microbubbles can enhance permeability of BBB and is