Decide if anything added to the agar is hazardous. Antibiotics, pesticides, blood, or other potentially harmful chemicals have special disposal guidelines. Treat plates with these additives as a biohazard. Sterilize agar with additives before disposing as solid waste. Choose the proper biohazard bag...
Explain the different microorganisms. Describe fungi, bacteria, and viruses from their history to modern biology. Define facultative anaerobes. How can they be (theoretically) distinguished in FTM tubes and by using Brewer's agar plates? How is facilitated diffu...
What is the value of petri plates in microbiology? What is bacterial culture in yogurt? Which pathogen (Staphylococcus epidermidis or E. coli) is able to grow on Blood Agar plate and why? Is this type of agar serving as differential, as selective, or as both? Explain why. ...
When sterilizing the inoculating loop, make sure that the entire loop turns orange before using on the agar plates. When streaking the agar with the loop, be sure to keep the loop horizontal and only streak the surface of the agar. If using sterile toothpicks, use a new toothpick when per...
(<30) do not give statistically reliable numbers, and overcrowded plates (>300 colonies) make it difficult to accurately count individual colonies. Also, counts in this range minimize occurrences of more than one bacterial cell forming a single colony. Thus, the calculated CFU is closer to the...
So the first thing we’re going to talk about is the experimental procedure and tools used for the experiment. Both changed more than a lot by the time I was finished, my original tools were, 12- 100x15 mm agar Petri plates, 12 disinfected swabs, 2 bottles of DI water, 12- 18x150mm...
An average of the two technical replicates (r) was used to make these calculations. One-way analysis of variance (ANOVA) was conducted to determine statistical significance between different RBC storage solutions and Tukey’s post-hoc test was conducted for a pairwise comparison when a significant...
Amongst bacteria, LABs are widespread in pickles, by cause of their ability to make high levels of lactic acid as well as being skilled to survive under high acidic conditions (Irkin & Songun, 2012). 3.1.1. Lactic acid bacteria (LABs) LABs are Gram-positive, catalase-negative, anaerobic,...
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This study focuses on developing a new and effective treatment for rotavirus infection using an extract from Saccharomyces cerevisiae, aiming to make this treatment easily accessible to everyone. 15 antigens and 26 antibodies were detected in serum and stool using ELISA. The titers of HRVq1, HRV...