demonstrate that Blimp1, which is required for primordial germ cell (PGC) specification, is dispensable for the derivation of embryonic stem cells (ESCs), epiblast stem cells (EpiSCs), and reprogramming. These findings argue that the acquisition of pluripotency does not require a Blimp1-dependent...
Nicole等发现PRDM1是一个在终末耗竭CD8+T细胞上调的阻遏蛋白。持续的刺激能够上调T细胞中PRDM1的表达,通过抑制PGC1a介导的代谢重编程改变了T细胞应对代谢应激。在CD8+T细胞中特异性敲除Prdm1基因能够使其抵御持续的刺激以及乏氧环境下所导致的功能丧失。 PRDM1与Treg细胞功能 过去有研究发现PRDM1高表达是Foxp3+Treg细胞...
Blimp1 marks unipotent embryonic luminal stem cells.aLineage tracing strategy adopted inb-d.Prdm1CreERT2mice are crossed toR26RmTmGreporter mice. Pregnant females were injected intraperitoneally with tamoxifen (TAM, 0.5 mg/25 g body weight) at embryonic day (E) E12.5, E14.5 or E17.5. ...
To investigate the effect of aspirin on mitochondrial functions, we detected MMP, a marker of early mitochondrial damage by JC-1-fluorescence staining, expression of PGC-1α, a marker of mitochondrial biogenesis by western blotting, and production of ROS by DCFH-DA kit in A549 and H1299 cells...
expenditure genes such as uncoupling protein (Ucp)s andPGC-1αwere upregulated in the skin ofp53TSD/−mice particularly the dermis/fat layer (Figures 3c–f). Because the elevated expression ofPGC-1αandUcp1are normally restricted to brown fat cells, we in the skin ofp53TSD/−mice ...
In day 11 opossum embryos, gene expression is most prominent in the genital ridges signifying the end of PGC migration. Because not all cells expressing Blimp1 are primordial germ cells, complications arise in inferring PGC location from Blimp1 staining. However, this study provides the first ...
We have now identified Blimp1, a potent transcriptional repressor of a histone methyltransferase subfamily, as a key regulator of PGC specification. Indeed, the unexpected early onset of Blimp1 expression in a few cells at the most proximal-posterior epiblast cells marks the origin of the germ ...
The differentiation of primordial germ cells (PGCs) is a fundamental step in development. PR domain-containing protein 14 (PRDM14) and B lymphocyte-induced maturation protein 1 (BLIMP1) play pivotal roles in mouse PGC specification. In the present study, we assessed the roles of chicken ...
To examine the molecular mechanism underlying this conversion, we focused on Blimp-1 and Akt, which are involved in PGC specification and dedifferentiation, respectively. Blimp-1 overexpression in embryonic stem cells suppressed the expression of downstream targets of the pluripotency network. Conversely,...
While PRDM1 and STELLA colocalized predominantly within the hindgut, where putative PGCs reside, other colocalizing cells were found in non-PGC sites. Additional PRDM1 and STELLA cells were found independent of each other throughout the posterior region, including the hindgut. The Prdm1-Cre-...