Suggested concentrations of blasticidin for selection in some examples of mammalian cells are listed below. Cell line Medium Blasticidin conc CHO (Chinese hamster ovarian cells) DMEM 5-10 μg/ml HEK293 (Human embryonic kidney cells) DMEM 5-15 μg/ml HeLa (Human uterine cells) DMEM 2.5-10 ...
For selection of Blasticidin-resistantE. coli,use Low Salt LB medium (10 g Tryptone, 5 g NaCl, 5 g Yeast Extract) containing 50-100 μg/ml Blasticidin. Depending on the bacterial strain used, optimize the Blasticidin concentration. If you get a lawn of bacteria on your Low Salt LB plate...
Blasticidin S deaminase gene from Aspergillus terreus (BSD): a new drug resistance gene for transfection of mammalian cells. Biochim. Biophys. Acta. 1219:653-9. Figures BlasticidinBack to the top Specifications Product concentration: 10 mg/ml Quality Control: Each lot is thoroughly tested to ...
Validated Application: Eukaryotic Selection⁄Stable Cell Line Generation, Bacterial Selection Form: Liquid Concentration: 10 mg⁄ml, 10 mg⁄mL, 10 mg⁄ml Quantity: 10 vials Sterility: Sterile 储存 Storage conditions: -5°C to -20°C (protect from light)Shipping conditions: Frozen...
What is the selection concentration of Blasticidin in mammalian cells? The working concentration of Blasticidin for mammalian cell lines varies between 1 - 10 μg/ml, with a few reported cases of up to 30 μg/ml. In an initial experiment we recommend to determine the optimal concentration of...
杀稻瘟菌素;Blasticidin S 10mg 650元 20mg 1000元 50mg 2400元 产品性状:白色结晶性粉末 产品参数: Purity...≥98.0%,用于细胞培养及动物实验 Product concentration...10 mg/ml Endot
The working concentration of blasticidin for mammalian cell lines varies from 1 to 10 μg/ml, in a few cases up to 30 μg/ml. In a starting experiment we recommend to determine optimal concentrations of antibiotic required to kill your host cell line. After treatment, cell death occurs rapi...
Product concentration:10 mg/mlQuality Control:Each lot is thoroughly tested to ensure the absence oflot-to-lot variation.Purity:≥ 95% (HPLC)Endotoxin level:< 1 EU/mgPhysicochemical characterization:pH, appearanceCell-culture tested:potency validated inblasticidin-sensitive andblasticidin-resistant mammal...
Wortmannin (Sigma) was used as positive control at a final concentration of 100 nM. Cells were incubated with compounds for 2 h at 37°C, 5% CO2. The medium was carefully removed from each well and cells were fixed by adding 100 μL of 4% PFA (Sigma). After a 10 min incubation at...
It is used as a selection agent in both mammalian cells and bacterial cells. The recommended working concentration ranges from 1 to 30 μg/mL depending on the cell line and 25–100 μg/mL for bacterial selection. Cell death occurs rapidly, and blasticidin-resistant stable mammalian cell lines...