Blasticidin Back to the top Specifications Appearance (form): Liquid or powder Appearance (color): Colorless (solution), white (powder) Product concentration: 10 mg/ml (solution) Buffer: HEPES pH: 7.1 - 7.5 (solution) Purity:≥ 95% (HPLC) Sterility: 0.2 um filter sterilized (so...
Cytoplasmic signaling includes an increase in intracellular calcium concentration and calcineurin/calmodulin-mediated dephosphorylation of NFAT (nuclear factor of activated T cells), allowing its nuclear translocation and binding to promoter regions of ADCC and ADCP relevant genes [1, 2]. Balance in Fc...
Product concentration:10 mg/ml Buffer:HEPES pH:6.0 - 7.0 Purity:≥ 98% (HPLC) Sterility:0.2 um filter sterilized Endotoxin level:< 5 EU/mg Working concentration:0.5 - 10 μg/ml (mammalian cells), 100 - 125 μg/ml(E. coli)
1 single tube that combines several selective antibiotics. ➔ Simple Add directly to the culture at a 1:250 dilution. ➔ Optimized concentration The concentration of antibiotics has been optimized to maximize their effectiveness for the maintenance of these cells. ...
美国InvivoGen Invivogen主要提供抗生素,转染试剂,细胞因子,细胞系,质粒等产品。抗生素涵盖了细胞培养、筛选全系列,如Blasticidin S,Hygromycin B, Phleomycin, Primocin, Zeocin等细胞分选常用抗生素。尤其以抗细胞支原体污染的抗生素Plasmocin™zui为出名,该抗生素因其卓越性能受到客户一致好评。
-Mammalia,cells:The working concentration of blasticidin for mammalian cell lines varies from 1 to 10µg/ml (ex HeLa(HEK293) B 16), in a few cases up to 30µg/ml (ex PC10) In a starting experiment we recommend to determine optimal concentrations of antibiotic required to kill your ...
(GOI), cloned downstream of the tet-inducible CMV-EF1-TRE promoter. GOI expression is induced upon addition ofdoxycycline, a synthetic analog of the tetracycline antibiotic, leading to the derepression of the CMV-EF1-TRE promoter. Low doxycycline concentration, ranging from 1 to 10 ng/ml, is ...
(GOI), cloned downstream of the tet-inducible CMV-EF1-TRE promoter. GOI expression is induced upon addition ofdoxycycline, a synthetic analog of the tetracycline antibiotic, leading to the derepression of the CMV-EF1-TRE promoter. Low doxycycline concentration, ranging from 1 to 10 ng/ml, is ...
Importantly, GSDMD links the canonical and non-canonical inflammasome responses with the pore formation leading to stress signals, such as cytosolic ion concentration imbalances (i.e. K+ efflux) and ATP release. These signals induce the activation of NLRP3 and CASP1-mediated IL-1β/IL-18 matura...