Leon-del-Rio A, Gravel RA: Sequence requirements for the biotinyla- tion of carboxyl-terminal fragments of human propionyl-CoA carboxylase alpha subunit expressed in Escherichia coli. J Biol Chem 269:22964, 1994.Leon-Del-Rio, A. and R. A. Gravel (1994). "Sequence requirements for the ...
Immobilize the biotin-labeled DNA/RNA target sequence onto the beads and incubate with the cell extract. You can then isolate the bound proteins using magnetic separation. You can elute off the protein for characterization or apply the whole solid-phase complex directly, e.g...
Immobilize the biotin-labeled DNA/RNA target sequence onto the beads and incubate with the cell extract. You can then isolate the bound proteins using magnetic separation. You can elute off the protein for characterization or apply the whole solid-phase complex directly, e...
Figure 4.2.3Nucleophilic attack of serine on the carbonyl group (C=O) of biotin-X, SSE results in the stableO-acylated derivative. In addition to histidine-x-serine, this stable intermediate can be formed in the presence of linear sequences of histidine-x-t...
Biotinylation, the process of covalently attaching biotin to a molecule of interest, can be achieved either chemically or enzymatically. In contrast to chemical biotinylation methods, enzymatic biotinylation allows biotin to be linked at exactly one residue within the recognition sequence of a protein, ...
Only one protein with a predicted transmembrane sequence and a C-terminal basic residue was identified: the hypothetical protein, C9orf46 homolog (IPI00136293), homologous to the protein predicted to be encoded by human chromosome 9, open reading frame 46. We have designated the protein, Plg-...
The catalytic rates of these chimeric proteins were determined through transport measurements and expression measured by cell-surface biotinylation. These studies show that the sequence of putative scaffolding domain TM6 is both necessary and ... SS Vollers 被引量: 4发表: 2013年 ...
The bicistronic expression system is based upon a polynucleotide which comprises a nucleic acid encoding a fusion protein made up of a selected protein and a biotinylation peptide, a nucleic acid sequence coding for an internal ribosome entry site and a nucleic acid sequence encoding a biotin ...
The sequences of the oligonucleotides used for the DNA affinity purifications can be found in Supplementary Table 1. Oligos were annealed in Annealing buffer (20 mM Tris pH 8, 100 mM NaCl, 2 mM EDTA, forward oligo 20 μM, reverse oligo 30 μM) by heating the mixture at ...
a, Hypothetical examples of proteins with varying proportions of their sequence exposed to the cytosol or lumen. The extent of labelling by cytosolic or lumenal baits should be directly related to proportion of the sequence, and hence lysine residues available for biotinylation, exposed to the ...