Biotin-tagged photoaffinity probeLigand-protein interactionVascular endothelial growth factor receptor-2VEGFR-2Chemical proteomics is a branch of proteomics in order to decipher protein function and mechanism at the level of directly observed protein-ligand(or small molecule)interactions by using chemical ...
To confirm that labeling was occurring in the biologically relevant compartment (the nucleus), we imaged cells transfected with HA-tagged miniTurbo-FKBP12F36V, and treated with DMSO, 1 µM Cpd 1, or 1 µM Cpd 1 + 10 µM (+)-JQ1. In the absence of Cpd 1, miniTurbo...
the co-immunoprecipitation assay can be further modified so that no capture antibody is needed. The resulting pull-down assay is typically performed with a tagged bait protein. In many applications, the tagged bait protein is a recombinant...
Results and Discussion We generated transgenic Arabidopsis plants that conditionally express HA epitope tagged HopF2-BirA*17 (~60 kDa; "membrane") and BirA* (~35 kDa; "soluble") under the control of a dexamethasone-inducible pro- moter. Consistent with previous findings17,18, BirA*-tagged...
A Capture Coupling Method for the Covalent Immobilization of Hexahistidine Tagged Proteins for Surface Plasmon Resonance Surface plasmon resonance (SPR) is a robust method to detect and quantify macromolecular interactions; however, to measure binding interactions, one compon... AJ Kimple,RE Muller,DP...
A far-Western blot uses a tagged protein other than an antibody to probe a mem- brane. The probe recognizes and binds to pro- teins on the membrane through protein:protein interactions. Thus a signal generated from a band on the membrane indicates the presence of a protein that interacts ...
Biotin-tagged fluorescent sensor to visualize 'mobile' Zn(2+) in cancer cells Authors:Fang, L., Trigiante, G., Kousseff, C. J., Crespo-Otero, R., Philpott, M. P., Watkinson, M. Journal:Chem Commun (Camb)(2018):9619-9622 ...
Dual-label TAMRA Biotin Alkyne probe can be readily incorporated into azide-tagged biomolecules through CuAAC ligation. For example, dual-labeled newly synthesized proteins, glycans, lipids, or DNA/RNA can be readily isolated by affinity purification and visualized by in-gel fluorescence....
Biotinylation reagents provide the "tag" that transforms the poorly detectable molecules into probes that can be recognized by a labeled detection reagent or an affinity-capture matrix. Once tagged with biotin or a hapten, a molecule of interest—such as an antibody, lectin, drug, polynucleotide,...
We noticed that the expression level of BirA dropped significantly when it was co-expressed with the Avi-tagged target protein. In addition, we found that the labeled protein gradually loses its biotin during storage, which was indicated by the decreased efficiency of its binding to streptavidin-...