Using this strategy may result in some polymerization of the peptide or pro- tein if the molecule has both carboxyls and primary amines on its surface. The extent of polymerization can be minimized by decreasing the amount of EDC and/or increasing the amount of the biotin reagent used in ...
Peptides, drugs and nucleic acids may also be used as the bait for capturing interacting proteins. These probes can also be used to detect protein interaction without the capture and enrichment step. In the EMSA or gel shift assay, ...
Results and Discussion We generated transgenic Arabidopsis plants that conditionally express HA epitope tagged HopF2-BirA*17 (~60 kDa; "membrane") and BirA* (~35 kDa; "soluble") under the control of a dexamethasone-inducible pro- moter. Consistent with previous findings17,18, BirA*-tagged...
Pull-down Assay of Biotin-labeled Histone peptides Binding Buffer 50 mM Tris pH 7.5 150-300 mM NaCl 0.05% NP-40 1.Thaw GST-tagged proteins on ice. Spin at 13K for 10 min, and carefully take proteins from the top of the solution. Avoid taking any Glutathione sepharose beads with ...
Peptides were eluted from the C18 column into the mass spectrometer using a linear gradient (5–80%) of ACN (Acetonitrile) at a flow rate of 375 μl/min for 1.5 h. The buffers used to create the ACN gradient were: Buffer A (98% H2O, 2% ACN, 0.1% formic acid) and Buffer B...
BTN131202 即用型GFP标签蛋白裂解液 GFP-Tagged Protein Lysate ARB12330 大鼠免疫球蛋白M(IgM)检测服务 Rat immunoglobulin m,IgM ELISA KIT BL0788 兔IgG抗原(干粉) 每支添加于100ml马铃薯葡萄糖琼脂培养基基础中。,用于酵母菌、霉菌的计数" NF-241 兔抗绵羊红细胞(溶血素) ...
HA Synthetic Peptide (Product # 26184) is available for use in neutralization and control experiments and to competitively elute HA-tagged fusion proteins from immobilized anti-HA affinity resin. Background/Target Information HA Tag (hemagglutinin tag) is an epitope tag that provides a method to ...
Various ligands, including antibody (fragments), peptides, aptamers and small molecules (such as folate) have been employed for the design of targeted nanomedicines [[16], [17], [18], [19]]. Biotin, as a water-soluble vitamin, is essential for normal cellular functions, growth and ...
Product Categories: peptide Mol File: Mol File BIOTIN-TAT (47-57) Chemical Properties form Solid color White to off-white Safety Information MSDS Information BIOTIN-TAT (47-57) Usage And Synthesis Uses Biotin-TAT (47-57), a biotin tagged TAT, is a transactivator of transcription. Biotin-...
The resulting Comet and Mascot search results were individually processed by PeptideProphet42, and peptides were assembled into proteins using parsimony rules first described in ProteinProphet43 into a final iProphet43 protein output using the Trans-Proteomic Pipeline (TPP; Linux version, v0.0 ...