The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging...
摘要: PROBLEM TO BE SOLVED: To provide a biotin-like peptide lipid which has a new structure and can be coated on a hydrophobic surface to impart a function releasing a protein used for cell cultures sustainedly from the surface of the material....
Results and Discussion We generated transgenic Arabidopsis plants that conditionally express HA epitope tagged HopF2-BirA*17 (~60 kDa; "membrane") and BirA* (~35 kDa; "soluble") under the control of a dexamethasone-inducible pro- moter. Consistent with previous findings17,18, BirA*-tagged...
Pull-down Assay of Biotin-labeled Histone peptides Binding Buffer 50 mM Tris pH 7.5 150-300 mM NaCl 0.05% NP-40 1.Thaw GST-tagged proteins on ice. Spin at 13K for 10 min, and carefully take proteins from the top of the solution. Avoid taking any Glutathione sepharose beads with ...
Peptides were eluted from the C18 column into the mass spectrometer using a linear gradient (5–80%) of ACN (Acetonitrile) at a flow rate of 375 μl/min for 1.5 h. The buffers used to create the ACN gradient were: Buffer A (98% H2O, 2% ACN, 0.1% formic acid) and Buffer B...
tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the ...
Using this strategy may result in some polymerization of the peptide or pro- tein if the molecule has both carboxyls and primary amines on its surface. The extent of polymerization can be minimized by decreasing the amount of EDC and/or increasing the amount of the biotin reagent used in ...
Biotinylation of proteins and peptides has been used for many years and has become a common way to biochemically tag targets of interest (1). One advantage is the very strong binding interaction with avidin that can be used to purify biotin-tagged proteins (2,3). It can also be used as...
1:1000 (1 µg/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (1) and transfected (2). V5 Tag Antibody (MA5-15253-BTIN) in WB Western blot analysis of V5-tagged proteins was performed by loading 15 µg of 293T cell lysates, fr...
Various ligands, including antibody (fragments), peptides, aptamers and small molecules (such as folate) have been employed for the design of targeted nanomedicines [[16], [17], [18], [19]]. Biotin, as a water-soluble vitamin, is essential for normal cellular functions, growth and ...