Three different types of cholesterol biosynthesis inhibitors, lovastatin, ketoconazole, and 25-hydroxycholesterol, showed their respective resistance against amphotericin B (AmB) cytotoxicity in Chinese hamster ovary (CHO)-K1 cells. The negative correlation between the acquisition of AmB resistance and the ...
During the incubation in LB broth containing amphotericin, 15 mM L-rhamnose was added to induce the decomposition of pEffector and pDonor-n by pCutamp. Subsequently, the cells were cultured in non-selective LB broth containing 10 g/L sucrose for 8 h at 37 °C. The cells were ...
there is immersing interest on isolation and characterization of novel macrolides. The clinically useful macrolides have been traditionally derived from the screening of natural sources, and still the isolation
Antimetabolite, 5-fluorocytosine, a nucleotide analog, is used in antifungal chemotherapy in combination with amphotericin B (Banerjee et al.2014). Another compound,l-proline analog known as Icofungipen (formerly BAY-108888 and PLD-118), is a known antifungal agent that reached the Phase II c...
It has also been shown to be a good partner when used in combination with amphotericin B (Trinconi et al., 2014), miltefosine (Trinconi et al., 2016) and meglumine antimoniate (Trinconi et al., 2017) in an established CL animal model. In many different lineages of human cancer cells...
Metal complexes of 1,10-phenanthroline-5,6-dione alter the susceptibility of the yeast Candida albicans to Amphotericin B and Miconazole Ergosterol biosynthesis is an oxy- gen dependent process and also requires NADPH which is synthesised in the aerobically respiring mi- tochondrion. ... A Eshwik...
(v/v) heat-inactivated FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin. MCF-10A normal breast cells were cultured with Mammary Epithelial Basal Medium, which contains bovine pituitary extract, hydrocortisone, human epidermal growth factor, insulin, gentamicin, and amphotericin-B. Both ...
Antifungal drugs used to treat IA, e.g., voriconazole and amphotericin B, are associated with undesired side effects, detrimental drug-drug interactions, long therapeutic regimens, and persistence of poor treatment outcomes (2–5). Compounding the difficulty of treating these infections, the ...
HEK293T cells were obtained from ATCC (CRL-3216) and cultured in DMEM supplemented with 10% FetalClone (VWR), 4.5 g/L glucose and L-glutamine, and 1% (w/v) penicillin-streptomycin-amphotericin B (Thermo Fisher Scientific). FreeStyle™ 293-F cells (HEK293F) were obtained from Thermo...
Caffrey P, Lynch S, Flood E, Finnan S, Oliynyk M (2001) Amphotericin biosynthesis in Streptomyces nodusus: deductions from analysis of polyketide synthase and late genes. Chem Biol 8:713–723 Article PubMed CAS Google Scholar Carata E, Peano C, Tredici SM, Ferrari F, Talá A, Corti ...