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et al. Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Cell 163, 759–771 (2015). Article CAS PubMed PubMed Central Google Scholar Pickar-Oliver, A. & Gersbach, C. A. The next generation of CRISPR–Cas technologies and applications. Nat. Rev. Mol. Cell ...
Nucleartransferagus cannot interbreed with extinct mammoths, and so she would not be classifiable as a part of the species M. primigenius. 7. Internodal (ISC): Membership is determined by organisms’ position between nodes on a phylogenetic tree. While Nucleartransferagus’ genome was taken ...
For bacteria co-culture, ISC-enriched cells (ENR + CV) were differentiated in ENR and ENR + CD as previously described. After 6 days of differentiation, cell clusters were isolated as previously described. The cell pellet was resuspended in basal culture medium and plated in suspension...
A key limitation of ELISA is the need of individual plates/wells for each antigen or antibody class to be tested. Moreover, the antigen is immobilized to the plate, which can hide epitopes or increase the background noise. For these reasons, ELISAs are not well suited to detect low ...
A novel cysteine-rich sequence-specific DNA-binding protein interacts with the conserved X-box motif of the human major histocompatibility complex class II genes via a repeated Cys-His domain and functions as a transcriptional repressor. J Exp Med. 1994;180:1763–74. Article CAS PubMed Google ...
Cellular processes such as metabolism, decision making in development and differentiation, signalling, etc., can be modeled as large networks of biochemical reactions. In order to understand the functioning of these systems, there is a strong need for ge
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