Haptoglobins (Hp) are a group of genetically polymorphic serum glycoproteins that migrate in the a2-globulin regions. They exhibit a high affinity for haemoglobin (Hb), forming exceedingly stable complex (Polnovski & Jayle, 1938) in vivo... N Yoshioka,MZ Atassi 被引量: 36发表: 0年 Compara...
THE oxygen affinity of haemoglobin is profoundly affected by the presence of low concentrations of organic phosphates, in particular 2,3-diphosphoglycerate (DPG). This phenomenon, which has now been widely recognized as a physiologically vital control mechanism, can be explained qualitatively by our ...
Interestingly, the Ehhmbp26 and Ehhmbp45 proteins could be related to the mechanism of iron acquisition in this protozoan, since they were secreted to the medium under iron-starvation conditions, and presented higher binding affinity for haem than for haemoglobin. In addition, both proteins were ...
Identification of the high and low affinity CO2- binding sites of human hemoglobin. Nature 1975; 256:759-761.PERRELLA, M.,J. V. KILMARTIN,]. FOGG and L. RoSSI-BERNARDI (1975): Identification ofthe high and low affinity C02-binding sites of human haemoglobin. Nature 256: 759-761....
Our biochemical studies indicate that HID2 binds Hb with higher affinity than HID1 and that the Hb tetramer is engaged by two Shr receptors. NMR studies reveal the presence of a third autonomously folded domain between HID2 and a heme-binding NEAT1 domain, suggesting that this linker domain ...
To probe the structure of these p22HBP protein complexes, docking, molecular dynamics and MM–PBSA methodologies supported by experimental NMR ring current shift data have been employed. The tetrapyrroles studied were found to bind murine p22HBP with the following binding affinity order: HEMIN > ...
In similar fashion, hemoglobin also has competitive binding affinity for cyanide (CN-), sulfur monoxide(SO), nitrogen dioxide (NO2), and sulfide(S2-), including hydrogen sulfide (H2S). All of these bind to iron in heme without changing its oxidation state, but they nevertheless inhibit ...
The oxygen-linked zinc-binding site of human haemoglobin. Zn2+ is known to increase the 02 affinity of human haemoglobin. Previous data suggested that Zn2+ exerts its effect by directly binding to haemoglobin, rat... JG Gilman,GJ Brewer - 《Biochemical Journal》 被引量: 39发表: 1978年 All...
Specific [6,7-(3)H]2-hydroxyestradiol-17 beta ([3H]2-hydroxyestradiol) binding is saturable and displays a high and low affinity binding component. The apparent dissociation constants, KD, are 4 +/- 2 x 10(-10) and 2 x 10(-6) M with 13 fmol and 2.6 pmol bound/mg of protein,...
The HpuB protein resembles TonB-dependent receptors and has been shown to be sufficient for binding of Hb at a low affinity by Neisseria but not for import of haem23. HpuA is expressed outside the cell, anchored to the outer membrane by its acylated amino terminus7. Although a direct ...