The purification procedure successfully removed several enzymes that could potentially interfere with assay procedures for ligase activity, i.e. ATPase, AMPase, inorganic pyrophosphatase, and bile acid-CoA thiolase. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis the purified ligase gave a ...
Bile acid CoA ligase (rBAL) is strongly inhibited by cis but not by trans isomers of monounsaturated very long chain fatty acids: Charles N Falany, Michelle Johnson, Dong-Ning He, Xiaowei Xei, Landon Wilson, Stephen Barnes, University of Alabama at Birmingham, Birmingham, AL; James B ...
Bile acid 7α-dehydroxylation requires a multistep bile acid-inducible (bai) regulon-encoded pathway44. This multistep bai-encoded pathway contains bile acid CoA ligase (baiB), bile acid 7α-dehydratase (baiE) and so on25,45. Thus, it would be of great interest to investigate the detailed...
N. et al. Molecular cloning and expression of rat liver bile acid CoA ligase. J. Lipid Res. 43, 2062–2071 (2002). Article CAS PubMed Google Scholar Shonsey, E. M. et al. Bile acid coenzyme A: amino acid N-acyltransferase in the amino acid conjugation of bile acids. Methods ...
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63] that involves the deconjugation of TCDCA by bile salt hydrolase (BSH), the 7α- or 7β-dehydroxylation of CDCA, the ligation of UDCA and coenzyme A (CoA) thioesters by bile acid CoA ligase (BAL), and the formation of TUDCA by the catalysis of bile acid CoA:amino acid N-...
Czuba B, Vessey DA (1982) The effect of bile acid structure on the activity of bile acid-CoA:glycine/taurine-N-acetyltransferase. J. Biol. Chem. 257(15); 8761–8765. CAS Google Scholar Dayal B, Ertel NH (1998) Rapid hydrolysis of bile acid conjugates using microwaves: retention of abs...
Bile acid-CoA ligase deficiency--a new inborn error of bile acid metabolism. Born at 27weeks gestation, a child of consanguineous parents of Pakistani origin required prolonged parenteral nutrition. She developed jaundice, with exte... CPK Chong,PB Mills,P Mcclean,... - 《Journal of Inherited...
that conducts transmembrane transporting of unconjugated primary bile acids like CDCA and CA (Gérard, 2013; Elkins and Savage, 1998). Then, thebaiBgene product, a bile acid CoAligase, activates the free bile acids by ligating them with CoA (Mallonee et al., 1992). Protein frombaiAcan ...
The bile acid-CoA ligase (choloyl-CoA synthetase, EC 6.2.1.7) was found to be tightly bound to the membranes of the endoplasmic reticulum, not easily disrupted (Vessey and Zakim 1977) but readily inactivated by proteases, suggesting that the active site was on the cytoplasmic side of the ...