For BCL-xL (Cell Signalling #2764), cleaved Caspase 3 (Cell Signalling #9661) and MCL-1 (Abcam #ab32087) IHC staining the Leica Bond Rx Autostainer was used. All FFPE sections underwent on-board antigen retrieval for 20 min using ER2 retrieval buffer (Leica, UK) before staining at a...
To balance a chemical equation, enter an equation of a chemical reaction and press the Balance button. The balanced equation will appear above. Use uppercase for the first character in the element and lowercase for the second character. Examples: Fe, Au, Co, Br, C, O, N, F. Ionic char...
The experimental studies are accompanied by quantum-chemical calculations, and we also analyse the possibility of further reductive dehydrogenation to give the double base-stabilized diboranes(4) [BH(-tbo)]2 and [BH(-tbn)]2 or oligomeric H2B(-tbn)2BH{BH(-tbn)2BH}nBH(-tbn)2BH2 (n = 0...
Intrinsic apoptosis is principally governed by the BCL-2 family of proteins, but some non-BCL-2 proteins are also critical to control this process. To identify novel apoptosis regulators, we performed a genome-wide CRISPR-Cas9 library screen, and it identified the mitochondrial E3 ubiquitin ligase...
To test the hypothesis that conversion of BAX from a closed-loop to an open-loop conformation is required to initiate direct BAX activation, we engineered a BAX construct that covalently enforced the native interaction between the α1-α2 loop and the α6 helix of BAX through disulfide bond ...
JS Binkley,LR Thorne - 《Journal of Chemical Physics》 被引量: 0发表: 1983年 Insights into the nature of SiH4-BH3 complex: Theoretical investigation of new mechanistic pathways involving SiH3 center dot and BH4 center dot radicals We have investigated a new type of interaction between silane (...
The animals were housed a maximum of three per cage in a temperature-controlled room (24 ± 2 °C), with 12–12 h light-dark cycle, humidity (60 ± 5%) and allowed to food and water ad libitum in the animal house of CSIR-Indian Institute of Chemical Biology, Kolkata. ...
ligand-induced decreases in peak intensity or signal disappearance. Another group of residues, including Q94, Q98, F157, L163, H165, and C166, exhibited focal, dose-responsive chemical shift changes. When mapped onto the structure of BAK (PDB ID: 2IMS34, Fig.5aand Supplementary Fig.6b), ...
Because the proposed “unlatching” model suggests that Bax activation disengages α5 from α6 (Figure 5B), tethering the two helices together by a disulfide bond might inactivate Bax. To test this hypothesis, we engineered a cysteine into α5 (V121C) and α6 (I136C) and introduced thes...
unperturbed by any panel of chemical agents. Those cells that were closest to the apoptotic threshold were therefore most primed for death and were predicted to be most responsive to chemotherapy generally, but without the ability to discriminate to which agent a cell was most likely to be most...