Enzyme-Linked Immunosorbent AssayThe main antigenic region of foot-and-mouth disease virus serotype C1, also called site A, has been inserted in zones of the beta-galactosidase important for the stabilization of the active site, causing important changes in the Km and the specific activity of ...
The enzyme is provided as a sterile-filtered solution in 20 mM Tris-HCl, 25 mM NaCl (pH 7.5). Specific Activity One unit of ß-(1-4)-galactosidase is defined as the amount of enzyme required to produce 1 µmole of p-nitrophenol (pNP) in 1 minute at 37°C pH 5 from p-nitroph...
Lac-z-gene produces beta-galactosidase enzyme, which is responsible for the colour of colonies. en.wikipedia.org These assays make use of a sugar linked to a dye which, when acted on by the enzyme beta-galactosidase, produces a characteristic color. ...
and various glycoproteins. It is an essential enzyme in the human body. Deficiencies in the protein can result in galactosialidosis or Morquio B syndrome. In E. coli, the gene of β-galactosidase, the lacZ gene, is present as part of the inducible system lac operon which is activated in...
galactosidaseenzymeactivity. Day1:Startovernightculturesinassaymedium.( 1 seecommentsbelow) Negativecontrol:cellslacking -galactosidase;positivecontrol:cellswithhighenzyme activity. Day2:Dilutecells1/100infreshmedium,growtomid-log. 1 Preparesolutions:Zbuffer, ...
Lactase Enzyme(Beta-galactosidase) Introduction Lactase (β-Galactosidase, EC 3.2.1.23), also known as β-galactosidase, hydrolyzes lactose to produce glucose and galactose. The lactase produced by the company is derived from Aspergillus oryzae and is a food grade lactase...
PURPOSE: To obtain the subject new enzyme having specific physicochemical properties, high in the transglucosylation ability to stevioside etc., capable of mass-producing a transglucosylation product as a raw material for various foods and medicines, by culturing Klebsiella bacteria. ;CONSTITUTION: Bacte...
What is the molecular weight of β-galactosidase (GLB1)? The molecular weight of GLB1 is 76 kD, although this protein is known to have multiple isoforms with lower molecular weight values. What is the function of GLB1? The common name of β-galactosidase is lactase. This enzyme is respons...
Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose intoAnti-Beta galactosidase抗体glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems...
Assay: Reporter Enzyme Compatible Cells: Yeast Cells Label Type: Enzyme Labeled Product Line: Pierce™ Substrate: ONPG Substrate Properties: Chemical Substrate Target Enzyme: Beta-Galactosidase Technique: Absorbance Detection Method: Colorimetric For Use With (Equipment): Microplate Reader ...