Helfand, S.L. and Naprta, B. (1996) The expression of a reporter protein, β- galactosidase, is preserved during maturation and aging in some cells of the adult Drosophila melanogaster. Mech Dev. 55:45-51.The expression of a reporter protein, betagalactosidase, is preserved during ...
These results suggest that the C-terminal region, in addition to the N-terminal alpha-helical structure, of beta subunit may be involved in the beta gamma complex formation. 展开 关键词: GLYCOSIDASE FAMILY 1 6-PHOSPHO-BETA-GALACTOSIDASE 4/7-SUPERFAMILY TIM BARREL X-RAY STRUCTURE DOI: 10.1006/...
TX14A does not stimulate GPR37L1 signaling or alter protein abundance. (a)β-galactosidase activity in Gpa1 or Gαs, Gαi1 or Gαi3 yeast chimera strains transformed to express either p426GPD vector or WT GPR37L1-eYFP. Transformants were stimulated with 10 µM TX14A for 24 h. n...
The cells were transfected with plasmids containing pCMV-beta-galactosidase, which was used to normalize the luciferase assay. Forty-eight hours after transfection, the cells were washed with PBS, dissolved in reporter lysis buffer (Promega, Madison, WI, USA), and harvested by scraping. Lysate ...
the biological function of Gpa1p and Ste2p constructs were quantified as a decrease of β-galactosidase activity due to the sequestration of Gβ/Gγ subunits with the expression of a functional Gα, and upon treatment of cells with α-factor, as an increase in the β-galactosidase activity ...
acting elements of the mouse pro alpha 1(II) collagen gene that control chondrocyte-specific expression in intact mouse embryos, we generated transgenic mice harboring chimeric constructions in which varying lengths of the promoter and intron 1 sequences were linked to a beta-galactosidase reporter ...
We have isolated the thermostable 9 kDa SaRD-protein from Sulfolobus acidocaldarius which exhibit RNase activity as well as DNA-binding properties (SaRD). The amino acid composition and the sequence of the 16 N-terminal amino acids show similarities to different RNases as well as to DNA-bindin...
To examine the potential utility of Ao38 cells, we measured the production of secreted human placental alkaline phosphatase (SEAP) and β-galactosidase expressed from recombinant AcMNPV baculoviruses. We also examined cell growth under various conditions and found that Ao38 cells adapted readily to ...
(as a reporter of injected cells’ progeny; Fig.3b,left,blue arrow) and detection of the β-galactosidase (β-gal) distribution were used to select and evaluate quantitatively those embryos with strongly unilateral expression (Fig.3b,right,blue arrowindicates high β-gal expression and, thus, ...
As Chn2 transcript was found to be robustly expressed in the cerebellum at all postnatal stages examined, we asked whether β-chimaerin played a functional role during cerebellar development. We took advantage of a previously generated knock-in mouse that expresses beta-galactosidase (βgal) from ...