(1) Beta-mercaptoethanol (βME). (2) Engineered Arabidopsis lines that exhibit beta-glucuronidase expression in the micropylar end of the seed, named Blue Micropylar End, BME lines. Bmf A pro-apoptotic protein (Bcl-2-modifying factor, 184aa). See Bcl-2 homology domains. BMPs Bone morphoge...
Samples (including AE preparations) were mixed with 5 × SDS loading buffer (containing E-mercaptoethanol), boiled at 95ºC for 5 min, and separated on 10% or 13% SDS-PAGE gels (13% was used for AE preparations). Proteins were transferred onto nitrocellulose membranes and blocked ...
Buffer: 8M urea, 20mM Tris-HCl, pH 8.0 containing 10mM beta-mercaptoethanol. Preservative: No products categories : Purified Enzymes products description : Beta-galactosidase, 114 kDa, enzymatically inactive. Control for Beta-gal fusion proteins. Does not react with human serum. ...
concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and ...
Institute (RPMI) 1640 medium containing 11.1 mM d-glucose and 2.1 mM l-glutamine supplemented with 10% heat-inactivated FBS, 2 mM l-glutamine, 10 mM HEPES (pH 7.2–7.5), 1 mM Sodium Pyruvate, Penicillin (100 units/ml), streptomycin (100 µg/ml) and 0.05 mM 2-mercaptoethanol (Sigma...
MIN6m9 cells were cultured in DMEM (GIBCO, Life Technologies) supplemented with 10% fetal bovine serum (FBSGIBCO), 1 g l À 1 D-glucose (Sigma Aldrich) and 5 ml l À 1 b-Mercaptoethanol (Sigma Aldrich). Islets were isolated from age-matched 4- to 5-month-old male C57B6 mice ...
Excised muscles were weighed and suspended in 20 volumes (w/v) of loading buffer containing 70 mM Tris-HCl (pH 6.7), 10% SDS, 10 mM ethylenediamine tetraacetic acid, and 5% β-mercaptoethanol. Suspensions were boiled for 5 minutes, left at room temperature for 10 minutes, and clarified ...
Embryoid bodies were fed every day from day 1 to day 3, then transferred to 6-well plates (Corning, St. Louis, MO, USA) in the differentiation media containing X-VIVO 15 media, 2 mM GlutaMAX, 50 U/mL of penicillin-streptomycin, 0.055 mM 2-mercaptoethanol, and supplemented with 50 ng...
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China) containing 11.2 mmol/l glucose and supplemented with 10% FBS (GE Healthcare Hyclone, Chicago, IL, USA), 1 mmol/l pyruvate, 10 mmol/l HEPES (Gibco, Life Technologies, Carlsbad, CA, USA), 100 U/ml penicillin, and 100 μg/ml streptomycin, 50 μmol/l 2-mercaptoethanol (Sigma-Al...