竟然打翻了beta-mercaptoethanol 整个房间就像一股炼煤厂的味甚至technician在门外就闻到臭我fri还说我怎么一股汽油味扩散到even我换了手套实验服都是臭的手机也是臭的 连刚刚放了个屁都是臭的 sds-page真的...
Dithiothreitol (二硫苏糖醇DTT), beta-mercaptoethanol (beta-me), andTris(2-Carboxyethyl)-Phosphine Hydrochloride (TCEPHCl) are sulfhydryl protective reducing agents. Reducing agents are typically used to prevent the oxidation of free sulfhydryl residues (cysteines) in the protein. Such oxidation can ...
(1) Beta-mercaptoethanol (βME). (2) Engineered Arabidopsis lines that exhibit beta-glucuronidase expression in the micropylar end of the seed, named Blue Micropylar End, BME lines. Bmf A pro-apoptotic protein (Bcl-2-modifying factor, 184aa). See Bcl-2 homology domains. BMPs Bone morphoge...
TCEP was used instead of β-mercaptoethanol in the lysis step. Furthermore, RNA was eluted in 40-μL RNase-free H2O instead of 60 μL, and the eluate was loaded onto the spin column a second time to retrieve higher RNA yield. RNA concentrations were measured using a Nanodrop 2000 (...
Beta-Propeller Protein-Associated Neurodegeneration BPAN is the only X-linked disorder of NBIA.136The disorder is due to X-chromosomal WDR45de novomutations. WDR45 belongs to a family of WD40 proteins that promote protein–protein interactions with a role in cell cycle control, translational regul...
Protein is >95% pure as determined by SDS-PAGE, by measuring optical density at 280 nm and by method of Bradford et al. Formulation Beta-Galactosidase (1mg/1ml) is formulated in 8M urea, 20mM Tris-HCl pH 8.0, and 10mM beta-mercaptoethanol ...
Bone marrow-derived macrophages (BMMs) were generated by flushing bone marrow cells from femurs and tibiae of mice. Cells were cultured in RPMI 1640 medium containing 15% (v/v) FBS, 2 mM L-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin, and 50 μM β-mercaptoethanol with 100 ...
Kinetic analyses of purified 3beta-HSD1 show that the Michaelis-Menten constants (Km) for substrates and cofactor are decreased dramatically (three- to eight-fold) by the addition of beta-mercaptoethanol (BME), which suggest that a disulfide bond may be critical to ligand utilization. Western ...
(1) Beta-mercaptoethanol (βME). (2) Engineered Arabidopsis lines that exhibit beta-glucuronidase expression in the micropylar end of the seed, named Blue Micropylar End, BME lines. Bmf A pro-apoptotic protein (Bcl-2-modifying factor, 184aa). See Bcl-2 homology domains. BMPs Bone morphoge...
Bound proteins were eluted by boiling at 95 °C for 5 minutes with 2xLDS sample buffer (Life Technologies, Grand Island, NY) containing 4 % β-mercaptoethanol. Immunoprecipitated or total protein was separated by SDS-PAGE on 4–12 % or 10 % Bis-Tris NuPage polyacrylamide gels (Life ...