BETA-KETOACYL-COA SYNTHASE GENES, PROTEINS AND THEIR USESMALKIT AZACHIAVI SADKAADA ZAMIR
Beta-Ketoacyl-acyl carrier protein (ACP) synthetase catalyzes the condensation reaction of fatty acid synthesis in Escherichia coli. The homogeneous enzyme reacts with hexanoyl-CoA to form hexanoyl-enzyme which was isolated and characterized. Hexanoyl-enzyme contains 2 mol of hexanoate/mol of enzyme ...
beta-Ketoacyl-acyl carrier protein (ACP) synthase III catalyzes the condensation of acetyl-CoA with malonyl-ACP in dissociated (Type II) fatty acid synthas... JT Tsay,W Oh,TJ Larson,... - 《Journal of Biological Chemistry》 被引量: 438发表: 1992年 Inhibition of fatty acid synthesis by...
FabG, an NADPH-dependent 3-ketoacyl reductase of Pseudomonas aeruginosa, provides precursors for... Examines contribution of FabG to coenzyme A (CoA) activity in Escherichia coli biosynthesis. Characterization of FabG as a 3-ketoacyl reductase of Pseudomo... Q Ren,Sierro,Nicolas - 《Journal of ...
pestis β-ketoacyl-acyl carrier protein synthases FabF and FabH, and compared these with the unpublished, deposited structure of Y. pestis FabB. Comparison of FabB, FabF, and FabH provides insights into the substrate specificities of these enzymes, and investigation of possible interactions with ...
Crystal structure of hexanoyl-CoA bound to β-ketoacyl reductase FabG4 of Mycobacterium tuberculosis Fab Gs, or beta-oxoacyl reductases, are involved in fatty acid synthesis. The reaction entails NADPH/NADH-mediated conversion of beta-oxoacyl-ACP (acyl-car... D Dutta,S Bhattacharyya,A Roychowdhur...
CoA esters served as substrates with the β-ketoacyl-ACP reductase and the enoyl-ACP reductase but were inert with β-hydroxyacyl-ACP dehydrase. These enzymes were inhibited by p-chloromercuribenzoate but not by N-ethylmaleimide. 展开 关键词: Hydro-Lyases Oxidoreductases Alcohol Oxidoreductases ...
Mechanistic studies on beta-ketoacyl thiolase from Zoogloea ramigera: identi- fication of the active-site nucleophile as Cys89, its mutation to Ser89, and kinetic and thermodynamic characterization of wild-type and mutant enzymes. Biochemistry 28, 5735-5742....
beta-ketoacyl-ACP reductase Beta-ketoacyl-ACP synthase Beta-Ketoacyl-ACP Synthase I beta-ketobutyric acid beta-ketobutyric acid beta-ketobutyric acid beta-ketobutyric acid beta-ketohydrogenase Beta-ketopropane Beta-ketopropane Beta-ketopropane beta-ketoreductase beta-ketothiolase Beta-ketothiolase defi...
A comparison of homologies of the domains of the multifunctional synthases that selectively use malonyl-CoA or methylmalonyl-CoA suggested that the acyltransferase (AT) and beta-ketoacyl synthase (KS) domains might be responsible for the substrate selectivity. To test this hypothesis, we expressed ...