beta-GalactosidaseNitrophenylgalactosidesEscherichia coli ProteinsCell ExtractsColorimetryCytological TechniquesStaining and LabelingTransfectionGene ExpressionProtein BiosynthesisWhen a transient or stable transfection assay is developed for a promoter, a primary objective is to quantify promoter strength. Because ...
Beta-galactosidase assay. 来自 NCBI 喜欢 0 阅读量: 112 作者: ST Smale 摘要: When a transient or stable transfection assay is developed for a promoter, a primary objective is to quantify promoter strength. Because transfection efficiency in such assays can be low, promoters are commonly fused ...
Assay: Reporter Enzyme Compatible Cells: Yeast Cells Label Type: Enzyme Labeled Product Line: Pierce™ Substrate: ONPG Substrate Properties: Chemical Substrate Target Enzyme: Beta-Galactosidase Technique: Absorbance Detection Method: Colorimetric For Use With (Equipment): Microplate Reader ...
Beta-Galactosidase In subject area: Medicine and Dentistry β-galactosidase is an enzyme that removes β-linked terminal galactosyl residues from substrates such as glycoproteins and glycosaminoglycans. From: Skin Aging Handbook, 2009 About this pageSet alert Also in subject areas: Nursing and Health ...
用于检测β-半乳糖苷酶(β-galactosidase)活性,是β-半乳糖苷酶的显色底物。经β-半乳糖苷酶催化后可产生黄色产物,在410-420nm波长可以检测吸光度。 2-硝基苯-beta-D-半乳糖苷上下游产品信息 上游原料 下游产品 2-硝基苯-beta-D-半乳糖苷 试剂级价格 ...
β - d -fucosidase can be separated from β - d -galactosidase by heat inactivation. A Marnay,R Got,P Jarrige - 《Experientia》 被引量: 20发表: 1964年 Beta-glucuronidase from Helix pomatia origin is not suitable for diosmetin analysis. Campanero et al. [1] have described an HPLC-MS/...
ONPG 2-Nitrophenyl β-D-galactopyranoside (ONPG) 2-Nitrophenyl beta-D-Galactopyranoside [Substrate for beta-D-Galactosidase] 物化性质 实验特性 LogP-0.70330 PSA145.20000 折射率-68.8 ° (C=1, H2O) 水溶性Soluble in water. 沸点572.4°C at 760 mmHg ...
应用 用于检测β-半乳糖苷酶(β-galactosidase)活性,是β-半乳糖苷酶的显色底物。经β-半乳糖苷酶催化后可产生黄色产物,在410-420nm波长可以检测吸光度。 储存温度 -20°C储存,充氩 运输条件 超低温冰袋运输 备注 如果有可能,您尽量在使用的当天配置溶液,并在当天使用完它。但是,如果您需要预先配制储备溶...
(2004). Quantitative β-galactosidase assay suitable for high-throughput applications in the yeast two-hybrid system. BioTechniques 36, 872-876.Möcli, N, Auerbach, D (2004) Quantitative β-galactosidase assay suitable for high-throughput applications in the yeast two-hybrid system. Biotechniques...
beta-galactosidase assay was done spectrophotometrically using O-nitrophenyl-beta-D-galactopyranoside (ONPG) as the substrate. Maximum beta-galactosidase activity of 2689 U/mg could be obtained at 72 h of fermentation using rice straw. beta-galactosidase activity was observed 1.369 fold with ...