beta-GalactosidaseNitrophenylgalactosidesEscherichia coli ProteinsCell ExtractsColorimetryCytological TechniquesStaining and LabelingTransfectionGene ExpressionProtein BiosynthesisWhen a transient or stable transfection assay is developed for a promoter, a primary objective is to quantify promoter strength. Because ...
Beta-galactosidase assay. 来自 NCBI 喜欢 0 阅读量: 123 作者: ST Smale 摘要: When a transient or stable transfection assay is developed for a promoter, a primary objective is to quantify promoter strength. Because transfection efficiency in such assays can be low, promoters are commonly fused ...
III. Staining Protocol (35 mm Dish Format) (1) List of Tables Table 1. Preparing X-Gal Staining Mix (2) I. Introduction The Beta-Galactosidase Staining Kit (Cat. No. 631780) provides a complete set of reagents for X-gal detection of beta-galactosidase expression in mammalian cells. ...
Negativecontrol:cellslacking -galactosidase;positivecontrol:cellswithhighenzyme activity. Day2:Dilutecells1/100infreshmedium,growtomid-log. 1 Preparesolutions:Zbuffer, phosphatebuffer,ONPG 2 . 1 Theassayisusuallyperformedoncellsinthemid-logphaseofgrowth.Onrichcarbon ...
C6 cells expressing ß-galactosidase were grown in 96-well plates. Cells were lysed and assayed using Thermo Scientific M-PER Mammalian Protein Extraction Reagent and Pierce ß-Gal Assay Reagent or corresponding Supplier X reagents. Reactions were stopped with 150µL of Stop Solution...
The Thermo Scientific Mammalian β-Galactosidase Assay Stop Solution is a component of the Mammalian β-Galactosidase Assay Kit, which provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity. The Assay Reagent and Kit comprise a simple and versatile ...
英文名称:Beta Galactosidase (β-Gal) Activity Assay Kit (Fluorometric) 上海维晟生物科技有限公司 联系电话:13916859711 电子邮件:tech@chinabiovision.com CB指数:58 网址:www.chinabiovision.com 相关信息:产品目录(2731)用户评价 英文名称:Beta Galactosidase (β-Gal) Activity Assay Kit (Fluorometric) ...
Beta Galactosidase (β-Gal) Activity Assay Kit (Fluorometric) 价格电议 起批量≥1 assays 最小起订电询 供货总量电询 发货地址上海市浦东新区 建议售价￥电议/assays 更新日期2025年01月23日 产品规格见优宁维官网 即时洽谈立即询价查看联系方式 收藏产品发送留言 ...
英文名字 Beta Galactosidase (β-Gal) Activity Assay Kit (Fluorometric) 供应商 Biovision 产品货号 K821-100 产品报价 ￥询价/100assays 产品说明书 点击查看 购买方式 银行转账、电汇、支票、现金,在线支付宝及网银支付,或直接与我们电话联系400-6800-868. ...
Thermo Scientific Yeast -Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions in-vivo using two-hybrid systems.Features of Yeast beta-Galactosidase Assay Kit: