In this study, we have characterized changes in senescence-associated (SA) β-gal staining in early and late passage cultures, cultures established from donors of different ages, virally immortalized cells, and tissue slices obtained from donors of different ages. The effects of different culture ...
The mechanism of senescence, permanent G1 arrest, is gradually becoming elucidated; however, the exact function of senescence remains unclear. A marker assay detecting the acidicsenescence-associated beta-galactosidase (saβgal) has been used as an indicator for cellular senescence. However, more rece...
Expression of senescence associated-β-galactosidase and P16 in the spleen of aged rats; 衰老相关-β-半乳糖苷酶和P16在老年大鼠脾内的表达及意义3) senescence associated β-galactosidase 衰老相关β半乳糖苷酶 1. The cytochemical staining of senescence associated β-galactosidase(SA-β-GAL)was applie...
EC 2.7.11.1, 1196aa) a dual-specificity kinase that regulates a signalling cascade activated by brassinosteroid binding that is involved in plant development, including expression of light- and stress-regulated genes, promotion of cell elongation, normal leaf and chloroplast senescence, and flowering....
To further determine BMP7-induced breast cancer cell senescence and the role of telomerase inhibition, β-Gal staining was carried for the β-galactosidase activity in MCF-7 cells treated with BMP7 for different periods of time. As shown in Fig. 3A, β-Gal positivity was observed in the enl...
B Representative staining for senescence-associated β-gal activity and qRT-PCR for CDKN2A (p16) and CDKN1A (p21) expression in HUVECs exposed to irradiation [IR] or treated with etoposide [ETOP]. Data is relative to the control for each independent experiment. C qRT-PCR of CDKN1A (p21) ...
In a murine model of pancreatic β cell cancer, genetic ablation of β1 integrin led to reduced tumor cell proliferation and senescence35. Pancreatic cancer cell lines overexpress integrin ɑ subunits 1–6 and β subunits; moreover, the β1 subunit is found to be constitutively active, ...
Cell lines and primary cultures from several teleost tissues and species were stained for senescence-associated beta-galactosidase (SA beta-Gal), revealing four general outcomes. (1) For long-standing fish cell lines that can be considered immortal, little or no SA beta-Gal staining was observed...
Quantifications showed a significant increase in both the average percentage of beta cells staining positive per islet between groups (p < 0.005; Figure 2A) and in the average percentages per mouse between groups (p < 0.05, p < 0.0005; Figure S2C). Similar to the senescence markers, there ...
The study elucidated the regulatory role of YY1 in the senescence process of beta cells. 2. Materials and methods 2.1. Reagents D-gal (Medchem Express; HY-N0210). Eudesmin (ChemFaces; CFN96266). H2O2 Solution (Sigma; 323381).SA-β-gal Staining Kit (Beyotime; C0602). Cellular Senescence...