Next, we evaluated the effects of the same cytokines on cellular senescence in patient-derived EOs in terms of the resulting expression levels of SA-β-gal. We subjected the specimens to fluorescence staining using a SPiDER-β-Gal assay kit and evaluated their fluorescence intensity via flow cyto...
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In this study, we reported that punicalagin treatment generated a senescent phenotype of BCPAP cells characterized as altered morphology, increased cell granularity and senescence-associated beta-galactosidase (SA-beta-Gal) staining. Senescence induced by punicalagin treatment was further confirmed by cell ...
Figure 2. Increased DSB and DDR are associated with cellular senescence in BRAP-deficient cells and cerebral cortices (A) Representative images of phospho-p53 immunofluorescence staining of WT and Brap−/− MEFs at P2. (B) Immunoblotting of total protein extracts from WT and Brap−/−...
Senescence-associated β-galactosidase staining Senescence-associated β-galactosidase staining was carried out according to the manufacturer's instructions (cat. no. 9860; Cell Signaling Technology, Danvers, MA, USA). Dissected tissue specimens were flash frozen in liquid nitrogen and immediately mounted...
(β-Gal) activity to determine the induction of senescence. Indeed, relative to responses observed in human B-ALL cells cultured in unconditioned medium and SCM, we observed significant increases in β-Gal activity in every human B-ALL cell line cultured in ACM (Fig.2d). Furthermore, ...
的BV2细胞活力的影响，并筛选出最佳的天麻素浓度；使用β-半乳糖苷酶（Senescence β-Galactosidase ，SA-β- Gal ）染色检测细胞衰老面积；使用生化法检测各组细胞活性氧（Reactive oxygen species ，ROS ）水平；使用 Enzyme linked immunosorbent assay （ELISA ）法检测各组细胞神经炎症因子IL-1β（Interleukin 1β...
and to explore the expression and production of caveolin-1 in H2O2-induced premature senescence.METHODS:The senescence of primary human umbilical vein endothelial cells (HUVECs) was induced by H2O2 as judged by morphology inspection,senescence-associated β-galactosidase (SA-β-Gal) staining and cell...
À and CD20 þ subpopulations was immunocytochemically stained with anti-FoxO3a, the localization of FoxO3a was different: FoxO3a was mostly localized in the nuclei of CD20 À cells Cellular Senescence in CD20 þ CD138 þ Subpopulation Cellular senescence was examined with SA-b-Gal staining...
Cell senescence was determined using the senescence β-galactosidase (SA-β-gal) staining kit (Cell Signaling) following the manufacturer’s instructions. At 24, 48, and 72 h post-transfection of SIRT4 plasmid, cells were fixed with a fixative solution for 15 min, followed by staining wit...