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We also failed to observe any differences in SA β-gal staining in skin cells in situ as a marker of aging in vivo. The level of cytochemically detectable SA β-gal was elevated in confluent nontransformed fibroblast cultures, in immortal fibroblast cultures that had reached a high cell ...
However, more recently, others have shown that expression of this low pH saβgal may not be solely limited to senescent cells. This study has extended the findings on stress induction of saβgal by assessing the enzyme activity in additional species (monkey and rat) and determining if saβ...
展开 关键词: enzyme preservation GMA histology methacrylate MMA parathyroid hormone-related peptide PTHrP X-gal staining DOI: 10.1080/10520290802127834 年份: 2008 收藏 引用 批量引用 报错 分享 全部来源 求助全文 Taylor & Francis 万方医学 Semantic Scholar dx.doi.org Ingenta 查看更多 相似文献 参考文献β...
To further determine BMP7-induced breast cancer cell senescence and the role of telomerase inhibition, β-Gal staining was carried for the β-galactosidase activity in MCF-7 cells treated with BMP7 for different periods of time. As shown in Fig. 3A, β-Gal positivity was observed in the enl...
Although the Escherichia coli lacZ gene, encoding beta-galactosidase (beta-gal), can be used as a standard reporter for monitoring the strength of a promoter or enhancer in a transient or stable transfection assay, it is predominantly used as an internal control during transient transfection ...
X-gal staining was used to check expression of β-gal. Results β-gal was expressed strongly and well-distributed in tumor in the first group, and stained blue in the liver, spleen, lung and kidney, but much weaker than that of tumor. In the second group, β-gal was expressed in the...
The SA-β-gal staining results of frozen tissue sections showed a significant increase in staining in the D-gal aging model group. Eudesmin exhibited a significant protective effect against pancreatic cell aging (Fig. 4A). Similarly, the immunofluorescence results of the aging-related cell cycle ...
We identified donor-derived areas of spermatogenesis in testes of recipients by staining with 5-bromo-4-chloro-3-indolyl β-D-galactoside (X-gal). GST pull-down. For GST pull-down assays, we lysed cells with RIPA buffer as described9 36 h after transfection. We purified GST fusion ...
Small dark-staining inactivated X chromosome seen in female (XX) cells. According to the Lyon hypothesis, which of the X chromosomes is inactivated is random. barrier-to-autointegration factor A conserved protein (BAF, breakpoint cluster region protein 1, 89aa) essential in proliferating cells. ...