beta-actin genefish cell linecell countingWe designed a universal primer set for quantitative polymerase chain reaction (qPCR) based on the nucleotide sequences of several fish beta-actin genes for counting cell number in small amounts of fish tissue. We also evaluated primer reactivity using...
qPCR引物对产品名称: 小鼠 14-3-3 beta qPCR引物对|14-3-3 beta qPCR primer pairs qPCR引物对靶点: YWHAB qPCR引物对适用种属: Mouse qPCR引物对GeneBank ID: qPCR引物对序列长度: 0 qPCR引物对保存条件: The lyop
The Applied Biosystems™ Mouse ACTB (actin, beta) Endogenous Control (VIC™ ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when it is used with other gene expression assays. Probe is labeled with VIC™...
The Applied Biosystems™ Mouse ACTB (actin, beta) Endogenous Control (FAM™ Dye⁄MGB Probe, Non-Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other TaqMan Gene Expression Assays. Probe is labeled with 6...
Real time PCR was performed using the following primers: Tert Probe: 5′ - /56-FAM/CACTGCGTA/ZEN/TAGCACCTGTCACCAA/31ABkFQ/ - 3′ Forward: 5′ GACTTCTTCCTGCACTTCCTG 3′; Reverse: 5′ CTTGTTCTCCATGTCTCCGA 3′ β - Actin Probe: 5′ - /56-FAM/CTGGCCTCA/ZEN/CTGTCCACCTTCC/31ABkF...
a Expression of TGFBI mRNA in asc-MDM, m1-MDM, and m2c-MDM analyzed by RT-qPCR in five different donors. b Detection of TGFBI protein in cell lysates by western blotting. β-Actin was used as loading control. Blots of three donors are shown. c TGFBI secretion of polarized MDM measured...
Fig. S1. E-Actin mRNA expression in AD (A) CT values of E-actin from qPCR of the hippocampus (HP), temporal cortex (TC), occipital cortex (OC), and cerebellum (CB) of control (white) and AD (black) brains. (B) E- Actin mRNA levels was normalized to the geometric mean ...
Oligonucleotides used as primers for qPCR and Sanger sequencing. Contributions T.S. and E.M. designed, performed and analyzed most of the experiments. T.S. wrote the manuscript. M.K. performed PET scans and together with T.S. and E.M. performed mice experiments. U.R., O.P. and N....
β-actin and U6 were used as the internal reference controls of CCDC88A and miR-199b-3p, respectively. The primers used are listed in Table 1. All experiments were performed in triplicate. Table 1 The primer sequences used for the RT-qPCR analysis. Full size table Small interfering RNA ...
Amaranthus tricolor L., a vegetable Amaranthus species, is an economically important crop containing large amounts of betalains. Betalains are natural antioxidants and can be classified into betacyanins and betaxanthins, with red and yellow colors, respe