从本研究的RT-qPCR结果中标准曲线和扩增曲线可以看出,实验体系和条件均具有特异性强、扩增效率高的特性,为以β-actin作为内参基因进行定量分析米蛾功能基因奠定了基础,为米蛾的分子生物学研究提供了基础资料。 【相关文献】 [1]BUSTIN S A.Quantification of mRNA using real-time reverse transcription PCR(RT-PCR...
The expression of NLRP3 was measured using qPCR/WB at 24 h intervals. 4.11. Immunohistochemistry We conducted the immunohistochemistry staining of tissue sections using the UltraSensitiveTM SP (mouse/rabbit) IHC Kit (MXB, Fuzhou, China), with specific primary antibodies including IL-1β-Abs (...