2002. beta- Actin and GAPDH housekeeping gene expression in asthmatic airways is variable and not suitable for normalising mRNA levels. Thorax 57:765-770.Glare E M, Divjak M, Bailey M J, et al. 2002. Beta-Actin and GAPDH housekeeping gene expression in asthmatic airways is variable and ...
Further studies exploring the function of actin in these environments will probably lead to a new integrative understanding of the roles of this housekeeping gene. View article B J.M. Lackie, in The Dictionary of Cell & Molecular Biology (Fifth Edition), 2013 β beta Entry prefix is given as...
Beta-actin (or β-actin) is a cytoskeletal actin isoform, highly conserved and ubiquitously expressed in eukaryotic organisms, making it a commonly used "housekeeping gene" in many laboratory techniques. It has been widely used as the internal control in RT-PCR and Western Blotting. The isotype...
Beta Actin Antibody Western Blot validation (1:5000 dilution) in porcine brain endothelial cells (Cat no:66009-1-Ig) FH Magda (Verified Customer) (12-31-2019) I needed to run a housekeeping gene control for my protein expression test. I was testing HEK293T cell lysates. My Lab did ...
BACKGROUND AND AIM OF THE STUDY: In gene expression studies, endogenous controls that are constitutively expressed (housekeeping genes) are commonly used to normalize for variations in cDNA synthesis efficiency. In the present study, a frequently used control gene, beta-actin, was examined in ovine...
Beyond its role as a housekeeping gene, it has been shown that beta-actin associates with endothelial nitric-oxide synthase (eNOS) and can therefore regulate nitric oxide activity in endothelial cells (PMID: 12734108). What are the post-transcriptional modifications (PTMs) to beta actin?
The housekeeping gene beta-actin (ACTB) was used as control. Two ETNA transgene-specific primer sets were used for PCR amplification with one set containing primer pairs 5′-AAACGCCAATTCCGACAT-3′ (forward) and 5′-GAAGGACCTCGAGTTACGC-3′ (reverse), and the other set containing primers ...
Gene expression was normalised to the housekeeping genes Gapdh and calculated using the delta Ct algorithm. The primer sequences are listed in Supplementary Table S6. Analysis of publicly available data ChIP-seq data of Tcf3 and Esrrb were downloaded from GEO (GSE11724 and GSE11431, respectively)...
signal. Relative quantification of gene expression was calculated using standard curves and normalized to GADPH in each sample. In order to confirm the best housekeeping gene we evaluated gene expression ofGADPH,18 sandactin.GADPHshowed a lesser CV (3%) than other genes (18s: 8.6% andactin: ...
Primers for nucleic acid amplification in detecting beta-actin and test method using these primers.It is intended to provide novel primers for nucleic acid amplification to be used in detecting mRNA of a housekeeping gene, more particularly, confirming the amplification of 尾-actin or GAPDH. ...